中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
6期
697-698
,共2页
李建伟%胡蕴玉%魏义勇%李丹%吕荣%白建萍%刘民
李建偉%鬍蘊玉%魏義勇%李丹%呂榮%白建萍%劉民
리건위%호온옥%위의용%리단%려영%백건평%류민
脂肪成体干细胞%分化%骨%组织工程
脂肪成體榦細胞%分化%骨%組織工程
지방성체간세포%분화%골%조직공정
Adipose-derived adult stem cells%Differentiation%Bone%Tissue engineering
目的 观察经成骨诱导后的脂肪成体干细胞(ADASCs)在体内的成骨分化行为.方法 分离培养兔ADASCs,用5-溴脱氧尿嘧啶核苷(BrdU)标记,并通过免疫组织化学方法检查标记结果.将标记的ADASCs体外成骨诱导培养2周后与脱钙骨基质(DBM)复合,共培养7d后植入自体肌袋内.术后8周取材,固定、脱钙、包埋后切片染色,镜下观察.结果 大量ADASCs被标记,标记率达(82.3±8.6)%;体外标记后的ADASCs可以向成骨方向分化;切片苏木素-伊红(HE)染色示体内有新生骨形成,切片BrdU免疫组织化学染色示新生骨处有阳性细胞.结论 ADASCs经体外成骨诱导后植入体内能够继续维持成骨分化,并进一步形成新生骨.
目的 觀察經成骨誘導後的脂肪成體榦細胞(ADASCs)在體內的成骨分化行為.方法 分離培養兔ADASCs,用5-溴脫氧尿嘧啶覈苷(BrdU)標記,併通過免疫組織化學方法檢查標記結果.將標記的ADASCs體外成骨誘導培養2週後與脫鈣骨基質(DBM)複閤,共培養7d後植入自體肌袋內.術後8週取材,固定、脫鈣、包埋後切片染色,鏡下觀察.結果 大量ADASCs被標記,標記率達(82.3±8.6)%;體外標記後的ADASCs可以嚮成骨方嚮分化;切片囌木素-伊紅(HE)染色示體內有新生骨形成,切片BrdU免疫組織化學染色示新生骨處有暘性細胞.結論 ADASCs經體外成骨誘導後植入體內能夠繼續維持成骨分化,併進一步形成新生骨.
목적 관찰경성골유도후적지방성체간세포(ADASCs)재체내적성골분화행위.방법 분리배양토ADASCs,용5-추탈양뇨밀정핵감(BrdU)표기,병통과면역조직화학방법검사표기결과.장표기적ADASCs체외성골유도배양2주후여탈개골기질(DBM)복합,공배양7d후식입자체기대내.술후8주취재,고정、탈개、포매후절편염색,경하관찰.결과 대량ADASCs피표기,표기솔체(82.3±8.6)%;체외표기후적ADASCs가이향성골방향분화;절편소목소-이홍(HE)염색시체내유신생골형성,절편BrdU면역조직화학염색시신생골처유양성세포.결론 ADASCs경체외성골유도후식입체내능구계속유지성골분화,병진일보형성신생골.
Objective To observe osteogenic differentiation of adipose-derived adult stem cells (ADASCs) after induction of osteogenesis in vivo.Methods ADASCs obtained from rabbits were cultured and labeled with BrdU.The labeling results were examined immunohistochemically.The labeled ADASCs were incubated in induced medium to induce osteogenesis.After 2 weeks,ADASCs were seeded onto deminerized bone matrix (DBM) and cultured for 7 days.The ADASCs/DBM complexes were transplanted into rabbit muscles.After 8 weeks,the complexes were taken out and retrieved,fixed,paraffin-embedded,and cut into 5 μm-thick sections.The sections were observed by staining.Results A great quantity of ADASCs were labeled,and the labeling rate reached (82.3±8.6)%.The labeled ADASCs in vitro could differentiate into osteoblasts.HE staining showed that there were new bones in vivo and BrdU immunohistochemical staining revealed that there were positive ADASCs in new bones.Conclusion After induction of osteogenesis in vitro,ADASCs can continue osteogenic differentiation in vivo,and form new bones further.