吉林省麻疹野病毒的基因型别和基因特征
길림성마진야병독적기인형별화기인특정
Genotype and gene charactrization of wild-type measles viruses circulated in Jilin Province
  • 万方数据
    中华实验和临床病毒学杂志 중화실험화림상병독학잡지
    CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
    2008年 6期 409-412 ,共4页

    陈超%许松涛%周剑惠%姬奕昕%侯祥%刘桂艳%王爽%常新%田鑫%李大强%刘影%许文波

    진초%허송도%주검혜%희혁흔%후상%류계염%왕상%상신%전흠%리대강%류영%허문파

    麻疹病毒%流行病学,分子%基因型 痳疹病毒%流行病學,分子%基因型
    마진병독%류행병학,분자%기인형
    Measles virus%Epidemiolngy,molecular%Genotype
    目的 阐明吉林省流行麻疹野病毒的基因型别和基因特征,为制定麻疹防控策略措施提供科学依据.方法 用逆转录-聚合酶链反应.限制性片段长度多态性分析(RT-PCR-RFLP)方法对2001-2006年分离的38株麻疹病毒进行基因分型;选择麻疹病毒代表株RT-PCR扩增N基因C-末端450个核苷酸,对比野毒和疫苗株核苷酸和氨基酸同源性,并构建N基因亲缘关系树.结果 经RT-PCR-RFLP基因定型,38株麻疹病毒分离珠均为H1基因型;对其中的29株进一步进行N基因C-末端450个核苷酸的序列测定和分析证实均为H1基因型的H1a亚型.吉林分离株与我国麻疹疫苗S191株450个核苷酸同源性为88.0%～89.4%,所提示氨基酸的同源性为91.8%～92.7%;吉林省内分离株核苷酸平均变异小于1.4%.结论 H1a基因亚型为吉林省近年来流行的麻疹野病毒绝对优势基因亚型.不同年份存在相同麻疹病毒的持续循环传播,同一年份也存有H1a亚型内不同病毒的共循环;与其他省之间存在相同麻疹病毒引起的传播链.
    목적 천명길림성류행마진야병독적기인형별화기인특정,위제정마진방공책략조시제공과학의거.방법 용역전록-취합매련반응.한제성편단장도다태성분석(RT-PCR-RFLP)방법대2001-2006년분리적38주마진병독진행기인분형;선택마진병독대표주RT-PCR확증N기인C-말단450개핵감산,대비야독화역묘주핵감산화안기산동원성,병구건N기인친연관계수.결과 경RT-PCR-RFLP기인정형,38주마진병독분리주균위H1기인형;대기중적29주진일보진행N기인C-말단450개핵감산적서렬측정화분석증실균위H1기인형적H1a아형.길림분리주여아국마진역묘S191주450개핵감산동원성위88.0%～89.4%,소제시안기산적동원성위91.8%～92.7%;길림성내분리주핵감산평균변이소우1.4%.결론 H1a기인아형위길림성근년래류행적마진야병독절대우세기인아형.불동년빈존재상동마진병독적지속순배전파,동일년빈야존유H1a아형내불동병독적공순배;여기타성지간존재상동마진병독인기적전파련.
    Objective To study the genotype and gene characterization of measles wild viruses circulated in Jilin provinces,and to provide scientific evidences for setting down controlling and preventing strategy and measures.Methods 38 strains of measles virus isolated in 2001-2006 were genotyped by RT-PCR-RFLP,some strains of measles virus in Jilin province were chosen for the phylogenetic analysis and for the homology analysis of nucleotide and amino acid sequences. Results All the 38 strains of measles virus were identified as H1 genotype by RT-PCR-RFLP,and 29 strains of them were identified further as H1a by sequence analysis. The homology of nucleofide was 88.0%-89.4% and the homology of amino acid was 91.8%-92.7% .The average diversity was less than 1.4% .Conclusion The measles virus of H1a genotype was the circulating virus within recent years in Jilin province.There were the same measles virus strains circulating and transmitting at different years and also the different H1a measles virus strains co-circulating at the same year.There were the same transmission chain caused by the same measles virus with other provinces.
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