CAJ | 학술논문

以16种重要的籼稻和粳稻栽培品种为材料，研究了影响农杆菌转化水稻频率的有关因素。结果表明：CC培养基是绝大多数水稻愈伤组织的最适诱导与继代培养基；添加2.5～5mg/L ABA可以有效地改善水稻愈伤组织的质量；籼稻愈伤组织所需的筛选剂浓度低于粳稻愈伤组织所需的浓度；根癌农杆菌EHA105菌株对水稻的转化效果优于LBA4404和AGL1菌株的效果；头孢霉素对农杆菌的抑制效果优于羧苄青霉素的效果；共培养后进行适当的干燥处理既可增强脱菌效果，又可提高转化频率。应用我们所优化的农杆菌转化技术体系，获得了10个品种的水稻转基因植株。
이16충중요적선도화갱도재배품충위재료，연구료영향농간균전화수도빈솔적유관인소。결과표명：CC배양기시절대다수수도유상조직적최괄유도여계대배양기；첨가2.5～5mg/L ABA가이유효지개선수도유상조직적질량；선도유상조직소수적사선제농도저우갱도유상조직소수적농도；근암농간균EHA105균주대수도적전화효과우우LBA4404화AGL1균주적효과；두포매소대농간균적억제효과우우최변청매소적효과；공배양후진행괄당적간조처리기가증강탈균효과，우가제고전화빈솔。응용아문소우화적농간균전화기술체계，획득료10개품충적수도전기인식주。
The factors influencing the frequency of rice transformation mediated by Agrobacterium have been investigated by using 16 commercially important indica and japonica rice cultivars or lines. The main results were as following: For most rice CC medium was the best for both callus initiation and subculture. With supplement of 2.5～5mg/L ABA the quality of calli can be improved. The concentrafion of selective agent for Indica rice callus was lower than that for japonica rice callus.Agrobacterium tumefaciens strain EHA105 was more efficient than LBA4404 and AGL1 for rice transformation. The inhibitive effect of cefotaxine to Agrobacterium was better than that of carbercillin. The partial desiccation treatment after co-cultivation was beneficial to inhibit the growth of Agrobacterium and increase transformation efficiency. A stable and efficient Agrobacterium-mediated transformation system has been established in ten different rice cultivars and fertile transgenic plants have been obtained.