CAJ | 학술논문

目的：观察扶正化瘀319方促进肝细胞白蛋白生成的机理及其拆方配伍意义。方法：胶原酶灌流法分离大鼠肝细胞，原代培养。将扶正化瘀319方拆分为扶正组、化瘀组、虫草组、丹参组、虫草加丹参组，灌胃大鼠，制备各组药物血清，并作用于培养肝细胞。ELISA法测定培养上清白蛋白含量，并以细胞层总蛋白量校正细胞数；RT-PCR法检测细胞前白蛋白原mRNA表达量。结果：药物血清对细胞形态无明显影响，对肝细胞白蛋白分泌及其前白蛋白原mRNA表达均有不同程度的促进作用，其中以扶正化瘀319方全方作用最为显著，而拆方组、扶正组与化瘀组的作用分别优于其中的单味药组--虫草组与丹参组。结论：促进肝细胞白蛋白基因表达及其蛋白合成是扶正化瘀319方升高慢性肝病血清白蛋白水平的主要机理。在促进白蛋白生成作用中，扶正与化瘀药物组合应用较单一治法或药物有综合优势。
목적：관찰부정화어319방촉진간세포백단백생성적궤리급기탁방배오의의。방법：효원매관류법분리대서간세포，원대배양。장부정화어319방탁분위부정조、화어조、충초조、단삼조、충초가단삼조，관위대서，제비각조약물혈청，병작용우배양간세포。ELISA법측정배양상청백단백함량，병이세포층총단백량교정세포수；RT-PCR법검측세포전백단백원mRNA표체량。결과：약물혈청대세포형태무명현영향，대간세포백단백분비급기전백단백원mRNA표체균유불동정도적촉진작용，기중이부정화어319방전방작용최위현저，이탁방조、부정조여화어조적작용분별우우기중적단미약조--충초조여단삼조。결론：촉진간세포백단백기인표체급기단백합성시부정화어319방승고만성간병혈청백단백수평적주요궤리。재촉진백단백생성작용중，부정여화어약물조합응용교단일치법혹약물유종합우세。
Aims:To investigate the mechanism of fuzheng Huayu 319 decoction and the compatibility of medicine in the decoction on albumin(Alb) production in hepatocytes. Methods:the hepatocytes were isolated through in situ liver perfusion with collagen from rats,and primarily cultured. Fuzheng Huayu 319 decoction(319 decoction) was divided into 5 subgroups:Fuzheng,Huayu,salvia miltiorrhizae,cordyceps,and salvia miltiorrhizae puls cordyceps. The drug sera were collected from rats that were feed on 319 decoction and its subgroups respectively, and incubated with cultured cells. The albumin(Alb) contents in culture medium were measured by ELISA, and were normalized by the total protein in the cell layer. The pre-Alb mRNA expression was analyzed by RT-PCR. Results:All drug sera had no obvious influence on the cell morphology,and promoted Alb secretion and pre-Alb mRNA expression in cultured hepatocytes in different degrees. Among them,319 decoction effect was the strongest,and the effects of Fuzheng and Huayu subgroup were better than respectively. Conclusion: the promotion of pre-Alb gene expression was the main mechanism of 319 decoction on Alb production. On this effect of 319 decoction,the combination of Fuzheng and Huayu drugs had comprehensive advantages over the single treatment group or single herb.