分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2001年
3期
249-252
,共4页
司帕沙星%亚硝酸%荧光光谱法
司帕沙星%亞硝痠%熒光光譜法
사파사성%아초산%형광광보법
研究了卤素(F,Cl,Br,I)与喹诺酮类药物司帕沙星反应产物的荧光特性,发现在酸性介质中,亚硝酸可氧化司帕沙星继而被卤素取代,所得产物具有强烈的荧光发射,其荧光强度随着卤素原子半径的增大呈现规律的增强效应。司帕沙星卤代物荧光较司帕沙星强58~151倍。据此建立了卤素增强荧光光谱测定司帕沙星的新方法。该方法用于直接测定尿液中司帕沙星的含量,结果令人满意。本文还对司帕沙星的荧光增敏机理进行了探讨
研究瞭滷素(F,Cl,Br,I)與喹諾酮類藥物司帕沙星反應產物的熒光特性,髮現在痠性介質中,亞硝痠可氧化司帕沙星繼而被滷素取代,所得產物具有彊烈的熒光髮射,其熒光彊度隨著滷素原子半徑的增大呈現規律的增彊效應。司帕沙星滷代物熒光較司帕沙星彊58~151倍。據此建立瞭滷素增彊熒光光譜測定司帕沙星的新方法。該方法用于直接測定尿液中司帕沙星的含量,結果令人滿意。本文還對司帕沙星的熒光增敏機理進行瞭探討
연구료서소(F,Cl,Br,I)여규낙동류약물사파사성반응산물적형광특성,발현재산성개질중,아초산가양화사파사성계이피서소취대,소득산물구유강렬적형광발사,기형광강도수착서소원자반경적증대정현규률적증강효응。사파사성서대물형광교사파사성강58~151배。거차건립료서소증강형광광보측정사파사성적신방법。해방법용우직접측정뇨액중사파사성적함량,결과령인만의。본문환대사파사성적형광증민궤리진행료탐토
The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensitites enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58~151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mchanism was proposed to explin this behavior.