CAJ | 학술논문

目的利用伯氏疏螺旋体基因工程抗原外膜蛋白C(OspC)建立间接ELISA，检测莱姆病特异性抗体IgM。方法基因工程抗原OspC的包被浓度和酶标抗μ链单抗所用浓度及血清稀释倍数，均由方阵滴定法确定，并进行精密度、特异性试验、阻断试验和干扰试验。结果 OspC最佳浓度为150 μg/L，批内平均变异系数4.6%，批间平均变异系数14.2%，用ELISA测定临床已确诊莱姆病33例，57例正常体检者，同时与进口ELISA试剂盒比较，两方法符合率97.8%。结论该方法特异性强、敏感性高、实验结果可靠，是莱姆病早期诊断的好方法。
목적이용백씨소라선체기인공정항원외막단백C(OspC)건립간접ELISA，검측래모병특이성항체IgM。방법기인공정항원OspC적포피농도화매표항μ련단항소용농도급혈청희석배수，균유방진적정법학정，병진행정밀도、특이성시험、조단시험화간우시험。결과 OspC최가농도위150 μg/L，비내평균변이계수4.6%，비간평균변이계수14.2%，용ELISA측정림상이학진래모병33례，57례정상체검자，동시여진구ELISA시제합비교，량방법부합솔97.8%。결론해방법특이성강、민감성고、실험결과가고，시래모병조기진단적호방법。
Objective To detect specific IgM of Lyme disease indirect ELISA using recombinant outer surface protein C(OpsC)of Borrelia burgdorferi in vitro was established. Methods Coated concentration of recombinant OspC and dilution multiple of serum anol concentration of enzyme secondary antibody were determined by block,and degree of percision.specificity interference and interruption test were performed. Results Best concentration of OspC was 150 μg/L.within-run CV was 6.3% between-run CV was 11.8%.Confimred 33 Lyme patients in clinic and 57 controls were examined meanwhile by this method and import ELISA kit,coincidena rate was 97.8%.Conclusion This ELISA using recombinant OspC was a good method for early diagnostic of Lyme disease.