CAJ | 학술논문

利用果蝇心脏发育基因zfh-1的cDNA序列和计算机克隆方法,在9号染色体上发现了一个新的人类同源基因.经国际基因命名委员会批准,命名为ZNF322.该基因总长度约为2.8 kb, 为一个连续的序列,编码一个402个氨基酸的蛋白质,与小鼠zfp-35编码的蛋白质最相似( 同源度为51.4%).该基因在人体肾、脑、结肠、胚胎心脏及黑色素瘤等多种组织中广泛表达, 其功能有待深入研究.
이용과승심장발육기인zfh-1적cDNA서렬화계산궤극륭방법,재9호염색체상발현료일개신적인류동원기인.경국제기인명명위원회비준,명명위ZNF322.해기인총장도약위2.8 kb, 위일개련속적서렬,편마일개402개안기산적단백질,여소서zfp-35편마적단백질최상사( 동원도위51.4%).해기인재인체신、뇌、결장、배태심장급흑색소류등다충조직중엄범표체, 기공능유대심입연구.
Based on the cDNA sequence of the zfh-1 g ene i n Drosophila, a novel human homolog located on chromosome 9 was found by com pute r cloning method. The gene is named ZNF322, which is approved by HUGO Gene N omeclature Committee. Its mRNA is about 2.8 kb and there is no intron in it. Th e gene encodes a protein of 402 amino acids,which is the best similar to the mou se protein encoded by zfp-35 (51.4%). Its ESTs appeared in many kinds nor mal tissues, such as kidney, brain, colon and fetal heart, and in melanoma o r other tumor tissues. The function of the gene remains to be studied.