CAJ | 학술논문

通过高温干烤和焦碳酸二乙酯(DEPC)两种技术除去核糖核酸(RNA)酶，在保证RNA的稳定性的情况下，在1．0%T,0%C的线性聚丙烯酰胺(即丙烯酰胺和双丙烯酰胺的总质量分数是1．0%，交联度为0%)筛分介质中，利用无胶筛分毛细管电泳技术对水稻中的总RNA进行了分析，整个分析过程在15 min内完成。利用5．0%T,0%C线性聚丙烯酰胺筛分介质，对水稻中的转移RNA(tRNA)进行了较为详细的分析，在30 min内可将tRNA分为两组共9个峰。该方法可用于植物中RNA的分析，具有快速、准确的特点。
통과고온간고화초탄산이을지(DEPC)량충기술제거핵당핵산(RNA)매，재보증RNA적은정성적정황하，재1．0%T,0%C적선성취병희선알(즉병희선알화쌍병희선알적총질량분수시1．0%，교련도위0%)사분개질중，이용무효사분모세관전영기술대수도중적총RNA진행료분석，정개분석과정재15 min내완성。이용5．0%T,0%C선성취병희선알사분개질，대수도중적전이RNA(tRNA)진행료교위상세적분석，재30 min내가장tRNA분위량조공9개봉。해방법가용우식물중RNA적분석，구유쾌속、준학적특점。
: When the RNase was eliminated through baking at 200 ℃ or treated by diethylpyrocarbonate(DEPC), the total RNA of rice could be separated within 15 min using 1．0%T, 0%C linear polyacrylamide as sieving matrix and 7 mol/L urea as denaturant． The tRNA of rice can be separated into two classes and about nine peaks when high concentrated polyacrylamide sieving matrix (5．0%T, 0%C) was used． This technique could provide one of the rapid and accurate methods for the determination of RNA in plants．