CAJ | 학술논문

以自东北地区分离的8株大豆慢生根瘤菌为供试菌用交叉凝集反应比较了它们与目前国内外已报道的15株大豆慢生根瘤菌标准血清型菌株之间的血清学关系。研究结果表明：（1）菌株93H10F5和HJ15不与供试标准血清型菌株发生交叉凝集反应，分别命名为O14 和O15 血清型。（2）菌株HJ19、HJ28、93F42与标准血清型菌株USDA94有交叉凝集反应，进一步的抗原吸收试验结果表明：HJ19和HJ28的抗原组成完全相同，命名为O3bcd血清亚型；U SDA9 4仅与HJ19有交叉反应，命名为O3ab血清亚型；93F42也与HJ19有部分共同抗原，命名为O3de 血清亚型。（3）菌株93HA8与标准血清型菌株USDA135有部分共同抗原，分别命名为O12 bc和 O12ab血清亚型。（4）标准菌株USDA127、USDA129与USDA123有交叉凝集反应，抗原吸收试验分别将其细分为O10ab，O10de和O10bcd等3个血清亚型。（5）将USD A的10个标准菌株重新命名为O1, O2, O4,O5, O6, O7, O8, O9, O11 和O13血清型。
이자동북지구분리적8주대두만생근류균위공시균용교차응집반응비교료타문여목전국내외이보도적15주대두만생근류균표준혈청형균주지간적혈청학관계。연구결과표명：（1）균주93H10F5화HJ15불여공시표준혈청형균주발생교차응집반응，분별명명위O14 화O15 혈청형。（2）균주HJ19、HJ28、93F42여표준혈청형균주USDA94유교차응집반응，진일보적항원흡수시험결과표명：HJ19화HJ28적항원조성완전상동，명명위O3bcd혈청아형；U SDA9 4부여HJ19유교차반응，명명위O3ab혈청아형；93F42야여HJ19유부분공동항원，명명위O3de 혈청아형。（3）균주93HA8여표준혈청형균주USDA135유부분공동항원，분별명명위O12 bc화 O12ab혈청아형。（4）표준균주USDA127、USDA129여USDA123유교차응집반응，항원흡수시험분별장기세분위O10ab，O10de화O10bcd등3개혈청아형。（5）장USD A적10개표준균주중신명명위O1, O2, O4,O5, O6, O7, O8, O9, O11 화O13혈청형。
The serological relationship between 8 strains of Bradyrhizobi um japonicum isolated from the north -east area of China and 15 standard serotype strains were compared with the cross-agglutination reaction method. It was found that str ains 93H10F5 and HJ15 showed no reactions with standard serotype strains tested and therefore, were classified as O14 and O15 new serotypes. Strains HJ19,HJ28,9 3F42 and USDA94 were further classfied as O3bcd,O3de and O3ab sub-serotypes by cr oss-absorption tests. Strains 93HA8 and USDA135 were also identified as O12 bc an d O12ab sub-serotypes.Standard serotype strains USDA127,USDA123 and USDA1 29 were further divided into O10ab,O10bcd and O10de sub-serotypes. M oreover,USDA31 and o ther 9 standard serotypes were renamed as O1, O2, O4, O5, O6, O7, O 8, O9, O11　and O13 serotypes,respectively.