CAJ | 학술논문

为研究昆山接骨片对体外成骨细胞骨形成功能的影响，探讨昆山接骨片促进骨折愈合的机理，分别用MTT法观察了含昆山接骨片Ⅰ号和昆山接骨片Ⅱ号的药物血清对成骨细胞增殖功能的影响，用PNPP法观察昆山接骨片Ⅰ号对成骨细胞碱性磷酸酶比活性的影响，以及用矿化结节计数法观察了昆山接骨片Ⅰ号对成骨细胞矿化功能的影响。成骨细胞增殖率测定结果显示，昆山接骨片Ⅰ号组为0.092±0.016，与对照组比较有显著性差异(P＜0.05)；而Ⅱ号组为0.230±0.026，与对照组比较无差异。ALP比活性测定结果显示，昆山接骨片Ⅰ号组为0.034±0.021，对照组为0.026±0.008，两组比较有显著性差异(P＜0.05))。矿化结节计数结果提示，昆山接骨片Ⅰ号组为25.833±7.782，对照组为13.333±6.153，两组比较有显著性差异(P＜0.05)。综合研究结果表明，以活血化瘀药物为主制成的昆山接骨片Ⅰ号具有促进成骨细胞的增殖、分化与矿化、成骨细胞的骨形成功能，从而发挥其促进骨折愈合的作用，单纯用螃蟹粉制成的昆山接骨片Ⅱ号则无此作用。
위연구곤산접골편대체외성골세포골형성공능적영향，탐토곤산접골편촉진골절유합적궤리，분별용MTT법관찰료함곤산접골편Ⅰ호화곤산접골편Ⅱ호적약물혈청대성골세포증식공능적영향，용PNPP법관찰곤산접골편Ⅰ호대성골세포감성린산매비활성적영향，이급용광화결절계수법관찰료곤산접골편Ⅰ호대성골세포광화공능적영향。성골세포증식솔측정결과현시，곤산접골편Ⅰ호조위0.092±0.016，여대조조비교유현저성차이(P＜0.05)；이Ⅱ호조위0.230±0.026，여대조조비교무차이。ALP비활성측정결과현시，곤산접골편Ⅰ호조위0.034±0.021，대조조위0.026±0.008，량조비교유현저성차이(P＜0.05))。광화결절계수결과제시，곤산접골편Ⅰ호조위25.833±7.782，대조조위13.333±6.153，량조비교유현저성차이(P＜0.05)。종합연구결과표명，이활혈화어약물위주제성적곤산접골편Ⅰ호구유촉진성골세포적증식、분화여광화、성골세포적골형성공능，종이발휘기촉진골절유합적작용，단순용방해분제성적곤산접골편Ⅱ호칙무차작용。
Objective:To study the effect of “Kunshan Jiegu” Tablet(KJT) onthe bone formation of osteoblasts.Methods:MTT method was used to observe the effects of blood serums of KJT-I and KJT-II on the proliferation of osteoblasts;PNPP method was used to evaluate the effect of KJT-I on the specific activity of alkaline phosphatase of osteoblasts;The counting method of mineralization nodules was used to observe the effect of KJT-I on osteoblasts' mineralization.Results:The effect of KJT-I group on the proliferation of osteoblasts is significantly different from that of the control group,while the result of KJT-II is on the contrary.The effect of KJT-I group on the activities of osteoblasts and mineralization differs greatly from that of the control group.Conclusion:KJT-I could increase proliferation,differentiation and minralization of osteoblasts,thus could inprove the fracture healing.