CAJ | 학술논문

采用生物发光方法检测重组的分支杆菌噬菌体对不同细菌的发光反应，并比较了仅在特定的温度范围内才能繁殖的温敏噬菌体Phage 88和正常噬菌体Phage 40对分枝杆菌感染活力测定时发光强度的差异，以建立用不同类型重组噬菌体检测结核分枝杆菌耐药性的方法和条件.结果显示两种噬菌体对各种分枝杆菌作用后均有发光，’对非分枝杆菌发光值很低，两者差异有显著性；不同的分枝杆菌发光值有差异：卡介苗的发光值最高，结核分枝杆菌的发光值最低；温敏噬菌体Phage 88的检测灵敏度大于正常噬菌体Phage 40，差异显著.因此可认为两株噬菌体均可特异地检测结核分枝杆菌，但Phage 88的效果优于Phage 40.
채용생물발광방법검측중조적분지간균서균체대불동세균적발광반응，병비교료부재특정적온도범위내 재능번식적온민서균체Phage 88화정상서균체Phage 40대분지간균감염활력측정시발광강도적차이，이건립용 불동류형중조서균체검측결핵분지간균내약성적방법화조건.결과현시량충서균체대각충분지간균작용후균유 발광，’대비분지간균발광치흔저，량자차이유현저성；불동적분지간균발광치유차이：잡개묘적발광치최고， 결핵분지간균적발광치최저；온민서균체Phage 88적검측령민도대우정상서균체Phage 40，차이현저.인차가 인위량주서균체균가특이지검측결핵분지간균，단Phage 88적효과우우Phage 40.
To compare the luminescent characters in different bacteria of two different recombinant mycobaceriaphages by using biolimenescent methods in order to understand the differences between sensitivity and specificity of these phages, and to set up methods to use recombinant mycobacteriaphages in detecting drug suscepbility of mycobacteria. Result showed that both two phages have high light production in action with mycobacterium selectively and have almost no light production with E. coli , the difference is very obvious. Among different mycobacterium, BCG has the highest light production and mycobacterium tuberculosis has the lowest light production. The sensitivity of Phage 88 is higher than Phage 40, the difference is obviously. It can be considered that both recombinant mycobacteriaphages can detect mycobacterium specifically, but Phage 88 is more suitable for clinical usage.