实用老年医学
實用老年醫學
실용노년의학
PRACTICAL GERIATRICS
2001年
1期
30-32
,共3页
严镭%刘国林%王树声
嚴鐳%劉國林%王樹聲
엄뢰%류국림%왕수성
小檗碱%老年性痴呆%血细胞凋亡抑制基因%肿 瘤坏死因子
小檗堿%老年性癡呆%血細胞凋亡抑製基因%腫 瘤壞死因子
소벽감%노년성치태%혈세포조망억제기인%종 류배사인자
目的 为研究小檗碱对老年痴 呆临床前期人群的防治意义及机制。 方法 采用系列〔痴呆 简易筛查量表(BSSD)、视觉保持试验(VRT)、数字广度测验(DS)等〕效度良好的神经心理学 量表,评定了阿尔茨海默病(AD)轻异组(30例)和脑血管性痴呆(VD)轻异组(24例)小檗碱干预 前后的认知功能,并应用放射免疫(RIA)法测定了血清肿瘤坏死因子(TNF-α)和酶联免疫吸 附测定法(ELISA)检测了全血细胞凋亡抑制基因(Bcl-2)的表达。 结果 服用小檗碱1疗程后,AD和VD轻异组BSSD等量表评定成绩提高,与治疗前比较 具有非常显著差异(PAD<0.02、PVD<0.01),治疗前AD和VD轻 异组血清TNFα和全血Bcl-2基因的表达均高于老年健康对照组(P<0.01),经治疗后 ,血清TNF-α含量下降和全血Bcl-2上调。 结论 提示小 檗碱可改善痴呆高危人群认知功能,可能与血清TNFα水平下降和全血Bcl-2基因表达上调 有关。对慢性脑病变神经细胞起保护作用。
目的 為研究小檗堿對老年癡 呆臨床前期人群的防治意義及機製。 方法 採用繫列〔癡呆 簡易篩查量錶(BSSD)、視覺保持試驗(VRT)、數字廣度測驗(DS)等〕效度良好的神經心理學 量錶,評定瞭阿爾茨海默病(AD)輕異組(30例)和腦血管性癡呆(VD)輕異組(24例)小檗堿榦預 前後的認知功能,併應用放射免疫(RIA)法測定瞭血清腫瘤壞死因子(TNF-α)和酶聯免疫吸 附測定法(ELISA)檢測瞭全血細胞凋亡抑製基因(Bcl-2)的錶達。 結果 服用小檗堿1療程後,AD和VD輕異組BSSD等量錶評定成績提高,與治療前比較 具有非常顯著差異(PAD<0.02、PVD<0.01),治療前AD和VD輕 異組血清TNFα和全血Bcl-2基因的錶達均高于老年健康對照組(P<0.01),經治療後 ,血清TNF-α含量下降和全血Bcl-2上調。 結論 提示小 檗堿可改善癡呆高危人群認知功能,可能與血清TNFα水平下降和全血Bcl-2基因錶達上調 有關。對慢性腦病變神經細胞起保護作用。
목적 위연구소벽감대노년치 태림상전기인군적방치의의급궤제。 방법 채용계렬〔치태 간역사사량표(BSSD)、시각보지시험(VRT)、수자엄도측험(DS)등〕효도량호적신경심이학 량표,평정료아이자해묵병(AD)경이조(30례)화뇌혈관성치태(VD)경이조(24례)소벽감간예 전후적인지공능,병응용방사면역(RIA)법측정료혈청종류배사인자(TNF-α)화매련면역흡 부측정법(ELISA)검측료전혈세포조망억제기인(Bcl-2)적표체。 결과 복용소벽감1료정후,AD화VD경이조BSSD등량표평정성적제고,여치료전비교 구유비상현저차이(PAD<0.02、PVD<0.01),치료전AD화VD경 이조혈청TNFα화전혈Bcl-2기인적표체균고우노년건강대조조(P<0.01),경치료후 ,혈청TNF-α함량하강화전혈Bcl-2상조。 결론 제시소 벽감가개선치태고위인군인지공능,가능여혈청TNFα수평하강화전혈Bcl-2기인표체상조 유관。대만성뇌병변신경세포기보호작용。
Objective To stud y the action mechanism and significance of berberin in preclinical dementia in t he aged. Methods A group of neuropsychological rating scales,i.e.bri ef s creening scale for dementia(BSSD),Benton visual retention test (VRT)and digit sp an(DS),with better reliability and valid ity were applied to evaluate the change of cognitive function in 30 cases of the preclinical Alzheimer’s disease(BSSD≥22 ~29 points called as PAD group)and 24 ca ses of the preclinical of vascular demen tial(BSSD≥22~29 points called as PVD gro up)before an d after the administration of berberin.W e used a number of rating scales above,s imulta neously serum tumor necrosis factor-α(TN F-α)and whole blood B cell lymphoma/leuk emia-2(Bcl-2)were measured by radioimmu noassay(RIA)and ELISA respectively. Results The cognitive functi on was greatly improved,with the score o f BSSD and other tests in PAD and PVD si gnifi cantly higher than those before the admi nistration of berberin(PAD<0.02,P VD <0.01).The whole blood Bcl-2 and the le vels of TNF-α in PAD and PVD were all si g nificantly higher than those in healthy and elderly control group before adminis tration.While after treatment Bcl-2 gene t ic expression was up-regulated and the l evel of serum TNF-α was declined. Conclusions All these findings sug gest that improving effect of berberin o n cognitive function in high risking peo ple of aged dementia may be related with whole blood Bcl-2 gene’s higher express ion and declining level of serum TNF-α w hich may protect the neurocytes of chron ic cerebropathy.