CAJ | 학술논문

为了研究两栖类变态过程中程序性死亡的分子机制，本文利用尾部离体培养、DNA含量分析、组织切片和形态学分析等方法，观察和分析了中华大蟾蜍变态过程中尾部程序性死亡的形态学变化；同时研究了磷脂酰胆碱特异性磷脂酶C的抑制剂对蝌蚪尾部程序性死亡的影响.发现这一特异性抑制剂明显阻止了中华大蟾蜍变态中尾部的萎缩，表明该酶在其尾部的程序性死亡中发挥重要作用.本文的这一发现为深入研究发育过程中程序性死亡的分子机制奠定了基础.
위료연구량서류변태과정중정서성사망적분자궤제，본문이용미부리체배양、DNA함량분석、조직절편화형태학분석등방법，관찰화분석료중화대섬서변태과정중미부정서성사망적형태학변화；동시연구료린지선담감특이성린지매C적억제제대과두미부정서성사망적영향.발현저일특이성억제제명현조지료중화대섬서변태중미부적위축，표명해매재기미부적정서성사망중발휘중요작용.본문적저일발현위심입연구발육과정중정서성사망적분자궤제전정료기출.
To understand the mechanism of programmed cell death during amphibian metamorphosis, we investigated the morphological changes during programmed cell death of the tadpole tail by tissue culture in vitro,DNA content analysis, transverse section assay and microphography. Simultaneously, the effect of the specific inhibitor of phosphatidylcholinespecific phospholipase C on the programmed cell death of tadpole tail was studied. We found that the specific inhibitor (D609, tricyclodecan-9-yl-xanthogenate) drastically blocked the programmed cell death of the tadpole. Our results first indicate that phosphatidylcholinespecific phospholipase C might play a important role in regulation of programmed cell death during Bu fo bufo gargarizans Cantor metamorphosis.