CAJ | 학술논문

目的：探讨重组人内皮抑制素对TNFα和IL-8介导的人血管内皮细胞株（endothelialcellofvessels,ECV）细胞增殖的抑制作用，并观察内皮抑制素对ECV表面粘附分子表达的影响。方法：用MTT法测定ECV的增殖;用间接免疫荧光法，在流式细胞仪上测定ECV表面粘附分子的表达。结果：内皮抑制素浓度在100～10000ng/ml时，能显著抑制ECV的增殖(P<0.01)，并抑制IL-8和TNFα介导的ECV增殖，且呈剂量依赖性关系。内皮抑制素在100～1000ng/ml时可抑制内皮细胞表面CD62E、CD40等粘附分子的表达。结论：内皮抑制素不但可抑制血管内皮细胞的增殖，控制肿瘤新生血管的形成，而且可抑制血管内皮细胞表面粘附分子的表达，对肿瘤的转移亦可能起抑制作用。
목적：탐토중조인내피억제소대TNFα화IL-8개도적인혈관내피세포주（endothelialcellofvessels,ECV）세포증식적억제작용，병관찰내피억제소대ECV표면점부분자표체적영향。방법：용MTT법측정ECV적증식;용간접면역형광법，재류식세포의상측정ECV표면점부분자적표체。결과：내피억제소농도재100～10000ng/ml시，능현저억제ECV적증식(P<0.01)，병억제IL-8화TNFα개도적ECV증식，차정제량의뢰성관계。내피억제소재100～1000ng/ml시가억제내피세포표면CD62E、CD40등점부분자적표체。결론：내피억제소불단가억제혈관내피세포적증식，공제종류신생혈관적형성，이차가억제혈관내피세포표면점부분자적표체，대종류적전이역가능기억제작용。
Objectives： The current study was designed to investigate the inhibitive effect of recombinant human endostatin on the proliferation of human endothelial cells of vessels (ECV) mediated by TNFα and IL-8. The influence of endostatin on the expression of adhesive molecules on the surface of ECV was also studied. Methods： The effect of endostatin on the proliferation of ECV in vitro was examined using MTT assay. The expression of adhesive molecules on the surface of ECV was determined by flow cytometry. Results： The proliferation of ECV was inhibited significantly after ECV was incubated with 100～10 000 ng/ml of endostatin for 72 h. The stimulating effect of TNFα and IL-8 on ECV was also suppressed markedly by endostatin. A dose-dependent inhibition was observed. At the level of 100～1 000 ng/ml, endostatin also inhibited significantly the expression of CD62E and CD40 on the surface of ECV. Conclusions： Endostatin not only exerts an inhibitive effect on the proliferation of endothelial cells, which may be helpful to the control of angiogenesis in solid tumors, but also suppresses the expression of adhesive molecules on the surface of endothelial cells. Thus, endostatin maybe play an important role in dealing with the metastasis of solid tumor.