CAJ | 학술논문

本工作研究了去细胞外钙对取自9天来亨鸡胚的培养肌管磷脂酰肌醇水解的影响.80 mmol/L K+暴露可以显著升高磷脂酰肌醇的水解.在暴露于无钙任氏液的肌管中,磷脂酰肌醇的水解随时间延长而呈指数递减,时间常数约为26分钟.在胞外无钙时,80 mmol/L K+ 引起的磷脂酰肌醇水解与对照(正常任氏液)相比略有下降.结果表明,高钾暴露能够增强培养肌管的磷脂酰肌醇的水解;但与成熟的肌纤维不同,细胞外钙是必须的.
본공작연구료거세포외개대취자9천래형계배적배양기관린지선기순수해적영향.80 mmol/L K+폭로가이현저승고린지선기순적수해.재폭로우무개임씨액적기관중,린지선기순적수해수시간연장이정지수체감,시간상수약위26분종.재포외무개시,80 mmol/L K+ 인기적린지선기순수해여대조(정상임씨액)상비략유하강.결과표명,고갑폭로능구증강배양기관적린지선기순적수해;단여성숙적기섬유불동,세포외개시필수적.
The effect of removal of external Ca2+ on phosphoinositide hydrolysis was investigated in cultured myotubes from 9-day-old Leghorn embryonic chicken. In the myotubes exposed to Ca2+ -free Ringer′s solution, the turnover of phosphoinositide was exponentially decreased with a time constant of about 26 min. In the presence of external Ca2+, the hydrolysis of phosphoinositide was significantly increased by exposure to 80 mmol/L K+ solution. After removal of external Ca2+, 80 mmol/L K+ exposure caused a slight decrease of phosphoinositides hydrolysis in comparison with the control (normal Ringer). It is indicated that hydrolysis of phosphoinositide in cultured myotubes can be enhanced by high K+ exposure. External Ca2+ is essential for this effect, which is different from mature muscle fibres.