植物生理与分子生物学学报
植物生理與分子生物學學報
식물생리여분자생물학학보
JOURNAL OF PLANT PHYSIOLOGY AND MOLECULAR BIOLOGY
2003年
1期
65-70
,共6页
杨玲%郑炳松%毛传澡%易可可%张卫萍%吴运荣%吴平%陶勤南
楊玲%鄭炳鬆%毛傳澡%易可可%張衛萍%吳運榮%吳平%陶勤南
양령%정병송%모전조%역가가%장위평%오운영%오평%도근남
水稻%种子根%伸长%cDNA-AFLP%旱作
水稻%種子根%伸長%cDNA-AFLP%旱作
수도%충자근%신장%cDNA-AFLP%한작
rice (Oryza sativa L.)%seminal root%elongation%cDNA-AFLP%upland condition
短期旱作促进水稻种子根的伸长.利用cDNA-AFLP技术分析种子根根尖在旱作条件下差异表达的基因,同时比较这些基因在种子根尖、侧根和不定根原基区的表达差异.在1 640个片段中,70个在种子根根尖中受旱作诱导,其中24个被克隆并测序.2个基因分别编码丙酮酸脱氢酶激酶(PDK)和腺嘌呤转磷酸核糖基酶(APRT),并用电子拼接技术获得水稻的APRT全长cDNA;另一个经cDNA末端快速扩增法延长后仍无同源序列.Northern杂交验证了这3个基因的cDNA-AFLP表达谱.这是首次报告使用cDNA-AFLP技术研究水稻根组织的差异表达基因.
短期旱作促進水稻種子根的伸長.利用cDNA-AFLP技術分析種子根根尖在旱作條件下差異錶達的基因,同時比較這些基因在種子根尖、側根和不定根原基區的錶達差異.在1 640箇片段中,70箇在種子根根尖中受旱作誘導,其中24箇被剋隆併測序.2箇基因分彆編碼丙酮痠脫氫酶激酶(PDK)和腺嘌呤轉燐痠覈糖基酶(APRT),併用電子拼接技術穫得水稻的APRT全長cDNA;另一箇經cDNA末耑快速擴增法延長後仍無同源序列.Northern雜交驗證瞭這3箇基因的cDNA-AFLP錶達譜.這是首次報告使用cDNA-AFLP技術研究水稻根組織的差異錶達基因.
단기한작촉진수도충자근적신장.이용cDNA-AFLP기술분석충자근근첨재한작조건하차이표체적기인,동시비교저사기인재충자근첨、측근화불정근원기구적표체차이.재1 640개편단중,70개재충자근근첨중수한작유도,기중24개피극륭병측서.2개기인분별편마병동산탈경매격매(PDK)화선표령전린산핵당기매(APRT),병용전자병접기술획득수도적APRT전장cDNA;령일개경cDNA말단쾌속확증법연장후잉무동원서렬.Northern잡교험증료저3개기인적cDNA-AFLP표체보.저시수차보고사용cDNA-AFLP기술연구수도근조직적차이표체기인.
The seminal root elongation of rice under short-duration upland condition was stimulated. cDNA amplified fragment length polymorphism (cDNA-AFLP) analysis was introduced to identify differentially expressed genes in seminal root tips under upland conditions, and to compare spatial expression in three root tissues-seminal root tips, lateral roots and adventitious root priomordia exposed to upland condition. Of the 1 640 fragments inspected, 70 showed an up-regulated expression pattern in seminal root tips under upland conditions. Twenty-four fragments were cloned and sequenced. Two fragments showed significant similarity to PDK and APRT. A full-length cDNA of rice APRT was obtained by electronic splicing. One cDNA extended by RACE showed no significant similarity to known sequences in the database. Northern blot analysis confirmed the expression of these three genes. This is the first report of the use of cDNA-AFLP to study differential gene expression in rice roots.