中国神经科学杂志
中國神經科學雜誌
중국신경과학잡지
CHINESE JOURNAL OF NEUROSCIENCE
2002年
4期
690-693
,共4页
单胺氧化酶B抑制因子%靛红%多巴胺%1-甲基-4-苯基-1,2,3,6-四氢吡啶
單胺氧化酶B抑製因子%靛紅%多巴胺%1-甲基-4-苯基-1,2,3,6-四氫吡啶
단알양화매B억제인자%전홍%다파알%1-갑기-4-분기-1,2,3,6-사경필정
monoamine oxidase type B inhibitor%isatin%dopamine%1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
研究内源性单胺氧化酶B抑制因子-靛红(2,3-吲哚醌,isatin)对MPTP引起的小鼠纹状体DA含量降低的影响.用高效液相色谱配电化学检测器测定纹状体DA,DOPAC和HVA水平.MPTP 30 mg/kg ip使纹状体DA,DOPAC和HVA含量与各自对照组比较显著降低(P＜0.01),预先靛红(200mg/kg@d,×4 d,ig)几乎完全抑制了上述效应,并减少了MPTP对DA更新率的升高,说明靛红有预防MPTP对中枢DA能神经元的毒性.
研究內源性單胺氧化酶B抑製因子-靛紅(2,3-吲哚醌,isatin)對MPTP引起的小鼠紋狀體DA含量降低的影響.用高效液相色譜配電化學檢測器測定紋狀體DA,DOPAC和HVA水平.MPTP 30 mg/kg ip使紋狀體DA,DOPAC和HVA含量與各自對照組比較顯著降低(P＜0.01),預先靛紅(200mg/kg@d,×4 d,ig)幾乎完全抑製瞭上述效應,併減少瞭MPTP對DA更新率的升高,說明靛紅有預防MPTP對中樞DA能神經元的毒性.
연구내원성단알양화매B억제인자-전홍(2,3-신타곤,isatin)대MPTP인기적소서문상체DA함량강저적영향.용고효액상색보배전화학검측기측정문상체DA,DOPAC화HVA수평.MPTP 30 mg/kg ip사문상체DA,DOPAC화HVA함량여각자대조조비교현저강저(P＜0.01),예선전홍(200mg/kg@d,×4 d,ig)궤호완전억제료상술효응,병감소료MPTP대DA경신솔적승고,설명전홍유예방MPTP대중추DA능신경원적독성.
The present study was designed to examine the effects of isatin ( indole-2,3-dione) , an endogenous selective monoamine oxidase type B ( MAO-B ) inhibitor, on the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ( MPTP ) -induced reduction of dopamine ( DA) and its metabolites in striatum of C57BL/6J mice. The amount of DA, dihydroxy-phenylacetic acid ( DOPAC ) and homovanillic acid ( HVA ) in striatum of C57BL/6J mice was measured by high performance liquid chromatography coupled with electrochemical detection. It was found that, administration of MPTP (30 mg/kg, ip)caused significant decrease of striatal DA,DOPAC and HVA ( P ＜0.01, compared with the control group) , while isatin ( 200 mg/kg @ d, × 4d, ig ) almost completely inhibited the MPTP-induced reduction of these parameters in the striatum,and reduced the increase of DA turnover induced by MPTP. In conclusion, isatin is able to prevent the depletive action of MPTP on central dopaminergic neurons, and therefore exerts a protective effect on the central dopaminergic system.
