CAJ | 학술논문

通过观察血红素氧化酶(HO)阻断剂--锌原卟啉(ZnPP)对肺组织、肺泡间质多形核白细胞数目、肺组织丙二醛含量和湿重干重之比的影响,并对肺组织HO活性和血内碳氧血红蛋白水平(COHb)进行检测,以探讨内源性HO/一氧化碳(CO)在肢体缺血再灌注(I/R)所致肺损伤中的作用.结果发现,大鼠双侧后肢I/R可导致急性肺损伤,同时使肺组织中HO活性和血内COHb水平显著升高;应用ZnPP预处理可使HO活性和COHb水平显著降低,但肺损伤却进一步加重.上述实验结果表明,肢体I/R致肺损伤时,肺组织中HO活性和内源性C0生成增多可减轻大鼠肢体I/R所致的肺损伤.
통과관찰혈홍소양화매(HO)조단제--자원계람(ZnPP)대폐조직、폐포간질다형핵백세포수목、폐조직병이철함량화습중간중지비적영향,병대폐조직HO활성화혈내탄양혈홍단백수평(COHb)진행검측,이탐토내원성HO/일양화탄(CO)재지체결혈재관주(I/R)소치폐손상중적작용.결과발현,대서쌍측후지I/R가도치급성폐손상,동시사폐조직중HO활성화혈내COHb수평현저승고;응용ZnPP예처리가사HO활성화COHb수평현저강저,단폐손상각진일보가중.상술실험결과표명,지체I/R치폐손상시,폐조직중HO활성화내원성C0생성증다가감경대서지체I/R소치적폐손상.
To investigate the role of endogenous heme oxygenase (HO)/carbon monoxide (CO) system in the lung injury as assessed by lung histology, polymorphonuclear count, malondialdehyde content and wet-to-dry weight ratio following ischemia-reperfusion (I/R) of hind limbs, zinc protoporphyrin (ZnPP) , an inhibitor of HO activity, was used, and the lung HO activity and blood carboxyhemoglobin (COHb) level were measured. The results showed that HO activity and COHb level were increased significantly and lung injury occurred after limb I/R. After administration of ZnPP, the lung injury was further aggravated while the HO activity and COHb level were significantly decreased. These findings suggest that upregulation of HO activity followed by subsequent CO production attenuates the lung injury induced by limb L/R in rats.