CAJ | 학술논문

该文以胡杨(Populus euphratica)悬浮细胞为材料,利用1.0%纤维素酶"onozuka"R-10、0.01%果胶酶Y-23、0.15%离析酶R-10和0.1%半纤维素酶的混合酶液消化细胞壁,得到原生质体.并利用膜片钳细胞贴附技术分别测到质膜内向和外向单通道电流.通道电流在不同水平上变化,表明并非只有一种类型的通道开放.树木细胞原生质体的分离以及利用膜片钳测定细胞质膜离子通道的成功实践为深入研究木本植物抗盐的细胞学机制奠定了基础.
해문이호양(Populus euphratica)현부세포위재료,이용1.0%섬유소매"onozuka"R-10、0.01%과효매Y-23、0.15%리석매R-10화0.1%반섬유소매적혼합매액소화세포벽,득도원생질체.병이용막편겸세포첩부기술분별측도질막내향화외향단통도전류.통도전류재불동수평상변화,표명병비지유일충류형적통도개방.수목세포원생질체적분리이급이용막편겸측정세포질막리자통도적성공실천위심입연구목본식물항염적세포학궤제전정료기출.
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1.0 % cellulase "onozuka" R-10, 0.01% pectolyase Y-23,0.15% macerozyme R-10 and 0.1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell-attached recording of patch-clamp technique.In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.