CAJ | 학술논문

目的观察bcl-2核酶对SMMC-7721细胞的作用,探讨bcl-2表达水平的改变对细胞端粒酶活性的影响.方法经脂质体介导的方法将PMTr-neo(正向bcl-2核酶真核表达载体)导入SMMC7721细胞中,细胞克隆转移扩大培养后,采用TUNEL,TRAP-PCR-ELISA，免疫组化技术结合图像分析技术检测SMMC7721/PMTr-neo细胞凋亡、细胞端粒酶活性及P16，P21的改变.结果较对照组SMMC7721/PMTr-neo细胞Bcl-2表达水平显著下降,伴有显著细胞凋亡现象；同时端粒酶活性下降，P16，P21表达水平显著增加.结论 bcl-2核酶可促进SMMC7721细胞发生凋亡,并降低细胞端粒酶活性，提示Bcl-2表达水平的改变对细胞端粒酶活性有一定的影响.
목적 관찰bcl-2핵매대SMMC-7721세포적작용,탐토bcl-2표체수평적개변대세포단립매활성적영향.방법 경지질체개도적방법장PMTr-neo(정향bcl-2핵매진핵표체재체)도입SMMC7721세포중,세포극륭전이확대배양후,채용TUNEL,TRAP-PCR-ELISA，면역조화기술결합도상분석기술검측SMMC7721/PMTr-neo세포조망、세포단립매활성급P16，P21적개변.결과 교대조조SMMC7721/PMTr-neo세포Bcl-2표체수평현저하강,반유현저세포조망현상；동시단립매활성하강，P16，P21표체수평현저증가.결론 bcl-2핵매가촉진SMMC7721세포발생조망,병강저세포단립매활성，제시Bcl-2표체수평적개변대세포단립매활성유일정적영향.
AIM To observe the effect of bcl-2 ribozyme on SMMC7721 cells andto investigate bcl-2 expression on cellular apoptosis and telomerase activity. METHODS PMTr-neo (sense bcl-2 ribozyme eucaryotic expression vector) was introduced into SMMC7221 cells by using lipofectin media-tion, after cloning TUNEL and TRAP in combination of ELISA and IHC in combination of imaging analysis was used to determine SMMC7721/PMTr-neo apoptosis and telomerase activity. RESULTS Compared with control, SMMC7721/PMTr-neo presented a significant drop in bcl-2 expression and an increase in P16 and P21 expression, accompanied by a decline in remarkable apoptosis and telomerase activity. CONCLUSION bcl-2 ribozyme promotes apoptosis of SMMC7721 cells and reduces telomerase activity. These provide a theoretical basis for applying antisense technique to treatment of hepatocarcinoma.