浙江预防医学
浙江預防醫學
절강예방의학
ZHEJIANG JOURNAL OF PREVENTIVE MEDICINE
2009年
7期
10-11,16
,共3页
铅%血铅%超氧化物歧化酶%丙二醛
鉛%血鉛%超氧化物歧化酶%丙二醛
연%혈연%초양화물기화매%병이철
Lead%Blood lead%Supemxide dismutase%Malondiatdehyde
目的 研究职业性铅接触者的氧化损伤,探讨血铅和氧化应激酶之间的变化关系,以探讨铅中毒的作用机制.方法 采用火焰原子吸收分光光度法测定空气中的铅含量,用石墨炉原子吸收分光光度法测定血铅浓度,选择超氧化物歧化酶(SOD)和丙二醛脱氢酶(MDA)作为氧化应激损伤的效应指标,分析血铅与氧化效应指标的变化.结果 共采集了车间内5个工作点的空气样品.接触组血铅浓度(244.274±124.59)μg/L,显著高于对照组(P<0.01);接触组SOD活性(61.27±6.97)KU/L,与对照组比较无统计学差异(P>0.05);接触组MDA含量(9.42±3.89)mmol/L,显著高于对照组(P<0.01);血清MDA含量与血铅之间存在正相关关系(r=0.3,P<0.01);吸烟、饮酒对SOD活性和MDA含量影响无统计学差异.结论 职业性铅接触引起人体血铅升高,作业场所空气中铅浓度与血铅含量变化不一致,血铅是近期铅接触者的敏感内接触指标,且血铅浓度越高,SOD活性代偿性升高;MDA含量越高,氧化损伤越明显.
目的 研究職業性鉛接觸者的氧化損傷,探討血鉛和氧化應激酶之間的變化關繫,以探討鉛中毒的作用機製.方法 採用火燄原子吸收分光光度法測定空氣中的鉛含量,用石墨爐原子吸收分光光度法測定血鉛濃度,選擇超氧化物歧化酶(SOD)和丙二醛脫氫酶(MDA)作為氧化應激損傷的效應指標,分析血鉛與氧化效應指標的變化.結果 共採集瞭車間內5箇工作點的空氣樣品.接觸組血鉛濃度(244.274±124.59)μg/L,顯著高于對照組(P<0.01);接觸組SOD活性(61.27±6.97)KU/L,與對照組比較無統計學差異(P>0.05);接觸組MDA含量(9.42±3.89)mmol/L,顯著高于對照組(P<0.01);血清MDA含量與血鉛之間存在正相關關繫(r=0.3,P<0.01);吸煙、飲酒對SOD活性和MDA含量影響無統計學差異.結論 職業性鉛接觸引起人體血鉛升高,作業場所空氣中鉛濃度與血鉛含量變化不一緻,血鉛是近期鉛接觸者的敏感內接觸指標,且血鉛濃度越高,SOD活性代償性升高;MDA含量越高,氧化損傷越明顯.
목적 연구직업성연접촉자적양화손상,탐토혈연화양화응격매지간적변화관계,이탐토연중독적작용궤제.방법 채용화염원자흡수분광광도법측정공기중적연함량,용석묵로원자흡수분광광도법측정혈연농도,선택초양화물기화매(SOD)화병이철탈경매(MDA)작위양화응격손상적효응지표,분석혈연여양화효응지표적변화.결과 공채집료차간내5개공작점적공기양품.접촉조혈연농도(244.274±124.59)μg/L,현저고우대조조(P<0.01);접촉조SOD활성(61.27±6.97)KU/L,여대조조비교무통계학차이(P>0.05);접촉조MDA함량(9.42±3.89)mmol/L,현저고우대조조(P<0.01);혈청MDA함량여혈연지간존재정상관관계(r=0.3,P<0.01);흡연、음주대SOD활성화MDA함량영향무통계학차이.결론 직업성연접촉인기인체혈연승고,작업장소공기중연농도여혈연함량변화불일치,혈연시근기연접촉자적민감내접촉지표,차혈연농도월고,SOD활성대상성승고;MDA함량월고,양화손상월명현.
Objective To study oxidative damage for occupational lead exposure, the relationship between serum lead and oxidative stress enzymes, and the mechanism of lead poisoning. Methods The lead content in the air was determined by flame atomic absorption spectrophotometric method, the lead concentration in serum was determined by graphite furnace atomic absorption spectrophotometric method. Superoxide dismutase (SOD) and malondialdehyde (MDA) as the effect indicators of oxidative stress were used to analyze the relationship between blood lead and the indicators. Results Five workplaces were monitored. The lead concentration in exposed group (244.27±124.59ug/L) was significantly higher than that in control group (P<0.01). The SOD activity in exposure group was 61.27±6.97KU/L not significantly different from that in control group (P>0.05), while MDA concetration in exposure group (9.42±3.89mmol/L) was significantly higher than in control group (P< 0.01). There was positive correlation between serum MDA and blood lead concentration (r = 0.3, P < 0.01) . The effects of smoking and drinking on the SOD activity and MDA content were not statistically significant. Conclusion Occupational lead exposure increases the blood lead level. It is inconsistent between the changes of lead concentration in workplace air and in blood lead. Blood lead is a sensitive indicator as the lead internal exposure. The higher blood lead level is, the higher the SOD activity and the MDA concentration, the more seriousthe oxidative damage is.
