CAJ | 학술논문

为了探索脂转移蛋白在蜡梅(Chimonanthus praecox)应对非生物胁迫过程中的可能功能,在构建蜡梅花cDNA文库及EST分析的基础上,通过对文库cDNA克隆全长测序,得到了3个蜡梅非特异性脂转移蛋白的基因(nsLTPs),分别命名为CpLTPI.1、CpLTPI.2和CpLTPI.3,GenBank登录号为FJ889521、FJ904082和FJ904083.它们的cDNA全长分别为611,1016和656 bp,ORF分别为360,360和351 bp,5'和3'端的非翻译区的长度不等,存在的调控基序有所不同.3个基因的编码蛋白均具有植物nsLTP的典型结构,即4对二硫键,4个α-螺旋,有1个可结合和容纳脂肪酸分子的类似口袋状的疏水结构,分子量约为9kD,为nsLTPI类.定量RT-PCR分析和比较蜡梅叶片中3个基因对非生物胁迫的应答情况,结果表明,3个nsLTP基因在蜡梅幼苗中的表达受干旱、ABA、低温和NaCl处理的影响程度不同,表明这些基因可能在蜡梅幼苗的水分平衡、耐受低温、离子代谢等过程中发挥着不同的作用.
위료탐색지전이단백재사매(Chimonanthus praecox)응대비생물협박과정중적가능공능,재구건사매화cDNA문고급EST분석적기출상,통과대문고cDNA극륭전장측서,득도료3개사매비특이성지전이단백적기인(nsLTPs),분별명명위CpLTPI.1、CpLTPI.2화CpLTPI.3,GenBank등록호위FJ889521、FJ904082화FJ904083.타문적cDNA전장분별위611,1016화656 bp,ORF분별위360,360화351 bp,5'화3'단적비번역구적장도불등,존재적조공기서유소불동.3개기인적편마단백균구유식물nsLTP적전형결구,즉4대이류건,4개α-라선,유1개가결합화용납지방산분자적유사구대상적소수결구,분자량약위9kD,위nsLTPI류.정량RT-PCR분석화비교사매협편중3개기인대비생물협박적응답정황,결과표명,3개nsLTP기인재사매유묘중적표체수간한、ABA、저온화NaCl처리적영향정도불동,표명저사기인가능재사매유묘적수분평형、내수저온、리자대사등과정중발휘착불동적작용.
To investigate the role of lipid transfer proteins (LTPs) in resistance of blossom of Chimonanthus praecox, three members of the LTPs family, namely CpLTPI.1, CpLTPI.2 and CpLTPI.3 (GenBank accession No.FJ889521, FJ904082 and FJ904083 ) were cloned respectively by randomly cloning and sequencing, based on the cDNA library constructed from Chimonanthus praecox flower and EST analysis. The three genes had full-lengths of 611, 1016 and 656 bp, respectively, with similar opening reading frame (ORF) of 360, 360 and 351 bp, and had different length of 5'and 3' untranslated region with different regulation motifs. The three genes, which belonged to the nsLTPI family, had the typical structure of the plant nonspecific lipid transfer protein including 4pairs of disulfide, 4 α-helices and 1 tunnel-like hydrophobic cavity which could contain lipid molecules. Quantitative RT-PCR analysis revealed that the three genes were differently regulated by drought, salinity, cold and ABA, which suggest that the three genes can play different roles in water balance, chilling tolerance, metabolism and other physiological processes in Chimonanthus praecox.