CAJ | 학술논문

本研究参考GenBank已发表的猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）Nsp2基因序列，在高致病性病毒Nsp2基因缺失区的两端保守区设计并合成了一对引物，建立并优化了能够区分经典PRRSV和高致病性PRRSV的RT-PCR诊断方法，并利用该方法对2007～2010年间江苏地区的45份可疑临床病料进行了检测。结果表明临床病料阳性率为40％，所有毒株均属于高致病性毒株。对PRRSV阳性病毒的Nsp2基因序列分析表明，所有18株PRRSV均属于美洲型毒株，与中国高致病性PRRSV代表毒株JXA1、WUH1的氨基酸同源性分别在82．2％～97．6％、80．4％～95.3％。此外，18株病毒的Nsp2共同存在不连续的30个氨基酸缺失，缺失位置与同期中国高致病性PRRSV具有相同的特征。通过本研究掌握了江苏地区2007～2010年间PRRSV流行情况及Nsp2基因变异特征，为地方猪繁殖与呼吸综合征的临床诊断和防治提供参考依据。
본연구삼고GenBank이발표적저번식여호흡종합정병독（Porcine reproductive and respiratory syndrome virus，PRRSV）Nsp2기인서렬，재고치병성병독Nsp2기인결실구적량단보수구설계병합성료일대인물，건립병우화료능구구분경전PRRSV화고치병성PRRSV적RT-PCR진단방법，병이용해방법대2007～2010년간강소지구적45빈가의림상병료진행료검측。결과표명림상병료양성솔위40％，소유독주균속우고치병성독주。대PRRSV양성병독적Nsp2기인서렬분석표명，소유18주PRRSV균속우미주형독주，여중국고치병성PRRSV대표독주JXA1、WUH1적안기산동원성분별재82．2％～97．6％、80．4％～95.3％。차외，18주병독적Nsp2공동존재불련속적30개안기산결실，결실위치여동기중국고치병성PRRSV구유상동적특정。통과본연구장악료강소지구2007～2010년간PRRSV류행정황급Nsp2기인변이특정，위지방저번식여호흡종합정적림상진단화방치제공삼고의거。
A RT-PCR to amplify the Nsp2 gene sequence of highly Pathogenic porcine reproductive and respiratory syndrome virus （PRRSV） was optimized with a pair of primers designed according to sequence published in GenBank. This RT-PCR method could differentiate classical PRRSVs and highly pathogenic PRRSVs. Clinical samples collected during 2007-2010 were detected with the RT-PCR and 40% samples（18/45） were positive for PRRSV, and all these viruses were similar to highly pathogenic PRRSV with deletion at Nsp2 region. The Nsp2 gene analysis revealed that the 18 strains belonged to North American genotype and the amino acids sequences showed 82.2%-97.6% and 80.4%-95.3% identity with JXA1 and WUH1 strains isol＇ated in China, respectively. The 30 amino acid discontinuous deletion was also found in the 18 positive viruses, which shared the same deletion position with the highly pathogenic PRRSV.