CAJ | 학술논문

以桉树品种‘3229’和‘U6’的木栓形成层为材料,比较分析了其从形成到脱落期间,3种抗氧化物酶(POD、SOD、APX)和IOX的活性,以及叶绿素、MDA、H2O2和DNA含量的动态变化.结果显示:随着2个品种木栓形成层衰老的加剧,叶绿素的含量均下降,但MDA含量、H2O2含量和LOX活力均增加.品种‘3229’木栓形成层成熟期的SOD活性显著高于形成期和脱落期,但形成期与脱落期的酶活性却无显著差异；品种‘U6’木栓形成层成熟期SOD的活性也为最高,但各时期间的SOD活性差异不显著.品种‘3229'和‘U6'的木栓形成层从形成期到成熟期APX活性均呈显著增加趋势,但成熟期后活性均又呈下降趋势.品种‘3229’木栓形成层的POD活性在成熟期到脱落期期间显著增加,而品种‘U6’却在形成期到成熟期显著增加.两品种DNA的动态分析电泳结果表明,桉树木栓形成层的脱落很可能包括细胞程序化死亡过程(PCD).
이안수품충‘3229’화‘U6’적목전형성층위재료,비교분석료기종형성도탈락기간,3충항양화물매(POD、SOD、APX)화IOX적활성,이급협록소、MDA、H2O2화DNA함량적동태변화.결과현시:수착2개품충목전형성층쇠로적가극,협록소적함량균하강,단MDA함량、H2O2함량화LOX활력균증가.품충‘3229’목전형성층성숙기적SOD활성현저고우형성기화탈락기,단형성기여탈락기적매활성각무현저차이；품충‘U6’목전형성층성숙기SOD적활성야위최고,단각시기간적SOD활성차이불현저.품충‘3229'화‘U6'적목전형성층종형성기도성숙기APX활성균정현저증가추세,단성숙기후활성균우정하강추세.품충‘3229’목전형성층적POD활성재성숙기도탈락기기간현저증가,이품충‘U6’각재형성기도성숙기현저증가.량품충DNA적동태분석전영결과표명,안수목전형성층적탈락흔가능포괄세포정서화사망과정(PCD).
During the period of senescence of eucalyptuses(Eucalyptus grandis × E.urophylla)cultivars ‘3229' and ‘U6' phellogen,a comparative study on changes of antioxidant enzymes,chlorophyll content,the contents of malondialdehyde(MDA),hydrogen peroxide(H2O2),activities of lipoxygenase(LOX)and DNA dynamic changes were conducted.Data showed that chlorophyll content decreased,but the contents of MDA and H2O2and activity of LOX increased in both cultivars '3229' and 'U6'.In'3229',the sample collected at the ripe stage of new phellogen formation(NPF)showed significantly high superoxide dis mutase(SOD)activity compared to that collected at the early and detached stage of NPF,while the latter two had no significant difference in enzyme activity.In ‘U6',however,no significant difference was found among the samplings for different batches,although the sample of the ripe stage of NPF displayed the highest SOD activity.Activity of ascorbate peroxidase(APX)significantly increased from the early to ripe stage of NPF and thereafter,declined.In ‘ 3229',peroxidase(POD)activity significantly increased between the ripe and detached stage of NPF,but in ‘U6',it did between the early and ripe stage of NPF.DNA ladders were also observed in the eucalyptuses phellogen.The results suggested that senescence in eucalyptuses phellogen could involve programmed cell death(PCD),reduced antioxidant activity and increased H2O2.