天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2012年
4期
481-485
,共5页
高速逆流色谱%celosin A%celosin B%蒸发光散射检测器
高速逆流色譜%celosin A%celosin B%蒸髮光散射檢測器
고속역류색보%celosin A%celosin B%증발광산사검측기
high-speed counter-current chromatography%celosin A%cdosin B%evaporative light scattering detection
连接有蒸发光散射检测器的高速逆流色谱仪首次成功的应用于制备和分离青葙子中的皂苷celosins A和B.二氯甲烷∶正丁醇∶甲醇∶水(4∶0.3∶3∶2)+0.5％冰醋酸作为洗脱溶剂系统.从半制备型HSCCC收集到的组分进行HPLC分析,可以得到:celosin A纯度为98.9％,celosin B的纯度为98.1％.这是高速逆流色谱仪首次被用于纯化青葙子中的皂苷,两个化合物的结构通过碳谱和质谱来确定.
連接有蒸髮光散射檢測器的高速逆流色譜儀首次成功的應用于製備和分離青葙子中的皂苷celosins A和B.二氯甲烷∶正丁醇∶甲醇∶水(4∶0.3∶3∶2)+0.5％冰醋痠作為洗脫溶劑繫統.從半製備型HSCCC收集到的組分進行HPLC分析,可以得到:celosin A純度為98.9％,celosin B的純度為98.1％.這是高速逆流色譜儀首次被用于純化青葙子中的皂苷,兩箇化閤物的結構通過碳譜和質譜來確定.
련접유증발광산사검측기적고속역류색보의수차성공적응용우제비화분리청상자중적조감celosins A화B.이록갑완∶정정순∶갑순∶수(4∶0.3∶3∶2)+0.5％빙작산작위세탈용제계통.종반제비형HSCCC수집도적조분진행HPLC분석,가이득도:celosin A순도위98.9％,celosin B적순도위98.1％.저시고속역류색보의수차피용우순화청상자중적조감,량개화합물적결구통과탄보화질보래학정.
High-speed counter-curent chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to preparative separation and purification of two saponins,celoeins A and B,which were from the main saponins of Semen celosiae by a one-step sepsration,using dichloromethane-methanol-n-butanol-water (4:3:0.3:2,v/v/v/v) with addition of 0.5％ glacial acetic acid as elution solvent.HPLC analysis of the fractions collected from the semi-preparative HSCCC of 100 ug of the main saponins showed that the purity of celosin A (30.5 mg)was 98.9％ and that of celosin B ( 10.7 mg) was 98.1％.The chemical structures of these components were eonfirmed by 13C NMR and EI-MS.
