CAJ | 학술논문

目的分析草酸铂处理后人结肠癌Lovo细胞系的差异表达蛋白,从蛋白质组学角度探讨草酸铂的作用机制.方法 Lovo细胞系按完全随机法分2组,即实验组(含草酸铂培养液培养)和对照组(无草酸铂培养液培养).培养后收集细胞依次进行二维电泳分离并选择差异点,采用质谱分析鉴定差异蛋白.结果两组双向电泳图谱分辨率高、重复性好,匹配率分别为80.16%(812/1013)和81.19%(829/1021).获取重复差异点22个,质谱分析其中10个,成功鉴定出与结肠癌相关6种蛋白:β2微球蛋白、剪接因子SFRS3、尿嘧啶DNA糖基化酶表达上调;60S核糖体蛋白P2、甘油醛3磷酸脱氢酶、鸟苷酸结合蛋白β2多肽样1表达下调.结论草酸铂作用人结肠癌Lovo细胞系后有多种差异蛋白表达,其与免疫增强、pre-mRNA转录修饰、DNA损伤修复、调控蛋白翻译及翻译后加工、能量代谢及信号传导通路等有关.
목적 분석초산박처리후인결장암Lovo세포계적차이표체단백,종단백질조학각도탐토초산박적작용궤제.방법 Lovo세포계안완전수궤법분2조,즉실험조(함초산박배양액배양)화대조조(무초산박배양액배양).배양후수집세포의차진행이유전영분리병선택차이점,채용질보분석감정차이단백.결과 량조쌍향전영도보분변솔고、중복성호,필배솔분별위80.16%(812/1013)화81.19%(829/1021).획취중복차이점22개,질보분석기중10개,성공감정출여결장암상관6충단백:β2미구단백、전접인자SFRS3、뇨밀정DNA당기화매표체상조;60S핵당체단백P2、감유철3린산탈경매、조감산결합단백β2다태양1표체하조.결론 초산박작용인결장암Lovo세포계후유다충차이단백표체,기여면역증강、pre-mRNA전록수식、DNA손상수복、조공단백번역급번역후가공、능량대사급신호전도통로등유관.
Objective To analyze differential expression proteins of human colon cancer Lovo cell line treated by oxaliplatin, and to explore the antitumor mechanism of oxaliplatin in the view of proteomics perspective. Methods The Lovo cells were randomly divided into two groups, including the experimental group (cultured in oxaliplatin- containing solution) and the control group (cultured in oxaliplatin-free solution). After culture, the both groups were processed by two-dimensional gel electrophoresis to select disparates, and differential proteins were identified by mass spectrometry analyses. Results High resolution and good reproducibility were observed on electrophoretograms of both groups, with matching rates at 80.16% (812/1013) and 81.19% (829/1021) respectively. Twenty-two repeated disparate points were obtained, ten of them were selected for mass spectrometry analyses. Six colorectal cancer related proteins were successfully identified, including 3 up-regulated proteins (β-2 microglobulin, splicing factor SFRS3 and uracil-DNA glycosylase) and 3 down-regulated proteins (60 S acidic ribosomal protein, glyceraldehyde-3-phosphate dehydrogenase and guanine nucleotide binding protein β polypeptide 2-like 1). Conclusion Multiple differential protein expressions are identified in human colon cancer Lovo cell line after oxaliplatin treatment, and the proteins are related to immune enhancement, pre-mRNA transcription modification, DNA damage repair, protein translation regulation, post- translation processing, metabolism of energy and signaling transduction pathway.