CAJ | 학술논문

目的了解肿瘤坏死因子-α(TNF-α)基因-308G/A多态性、血清水平与广东地区精神分裂症的相关性.方法用聚合酶链反应一限制性片段长度多态性技术检测155个核心家系精神分裂症患者及其父母外周血的TNF-α基因启动子区域-308G/A位点基因多态性;采用酶联免疫吸附法测定血清TNF-α水平.结果 TNF-α基因-308位点变异的G/A基因型频率在精神分裂症组中比父母对照组明显升高(27.1%;15.0%,P<0.01),血清TNF-α水平也显著高于对照组[(197.3±83.4)pg/ml;(95.2±46.8)pg/ml,P<0.05)].结论 TNF-α基因-308位点G/A多态性和血清TNF-α水平增高与精神分裂症发生相关联,支持TNF-α基因是精神分裂症的候选基因.
목적 료해종류배사인자-α(TNF-α)기인-308G/A다태성、혈청수평여엄동지구정신분렬증적상관성.방법 용취합매련반응일한제성편단장도다태성기술검측155개핵심가계정신분렬증환자급기부모외주혈적TNF-α기인계동자구역-308G/A위점기인다태성;채용매련면역흡부법측정혈청TNF-α수평.결과 TNF-α기인-308위점변이적G/A기인형빈솔재정신분렬증조중비부모대조조명현승고(27.1%;15.0%,P<0.01),혈청TNF-α수평야현저고우대조조[(197.3±83.4)pg/ml;(95.2±46.8)pg/ml,P<0.05)].결론 TNF-α기인-308위점G/A다태성화혈청TNF-α수평증고여정신분렬증발생상관련,지지TNF-α기인시정신분렬증적후선기인.
Objective To explore relationship of G308A polymorphism in the tumor necrosis factor a (TNF-α) gene and TNF-α serum level with schizophrenia. Methods The G308A polymorphism in TNFa was tested by PCR-based RFLP in 155 schizophrenic family trios from Guangdong Province by polymerase chain reaction and restriction fragment length polymorphism techniques, and level of TNF-alpha in serum by ELISA. Results Haplotype relative risk revealed that the transmitted A allelic frequency in patients was significantly higher than that in their parents (27.1% vs 15.0%, P< 0.01), and serum level of TNF-alpha in patients was also higher than that in their parents [(197.3±83.4) pg/ml vs (95.2±46.8) pg/ml, P<0.05)]. Conclusions There is rela-tionship of the TNF-α promoter polymorphism at position -308 and TNF-α serum level with schizophrenia,indica- ting that it is the candidate gene of schizophrenia.