CAJ | 학술논문

目的探讨RGC-32和E-cadherin蛋白在胰腺癌组织中的表达,分析其临床病理学意义以及两种蛋白表达之间的相关性.方法采用免疫组织化学SP法检测42例胰腺癌、12例慢性胰腺炎和8例正常胰腺组织中RGC-32和E-cadherin蛋白的表达.结果 RGC-32主要表达于胰腺腺泡细胞胞质内；E-cadherin主要表达于正常胰腺和慢性胰腺炎组织胰腺腺泡细胞胞膜,而在胰腺癌中则可出现胞质表达和(或)表达减弱等异常表达.胰腺癌组织中RGC-32的阳性表达率以及E-cadherin异常表达率分别为78.6％( 33/42)和54.8％ (23/42),均显著高于正常胰腺组织的37.5％ (3/8)和0以及慢性胰腺炎组织41.7％(5/12)和8.3％(1/12)(P值均＜0.05).胰腺癌组织RGC-32表达与肿瘤淋巴结转移和TNM分期有关(P值分别为0.016、0.025),而与患者的年龄、性别及肿瘤分化程度无关(P值分别为0.831、1.000、0.629)；E-cadherin异常表达与胰腺癌分化程度、淋巴结转移和TNM分期有关(P值分别为0.024、0.004、0.004),而与患者年龄、性别无关(P值分别为0.970、1.000).RGC-32的阳性表达率与E-cadherin异常表达率呈正相关(r=0.458,P＜0.01).结论胰腺癌组织中RGC-32阳性表达率及E-cadherin异常表达率均显著升高,RGC-32可能通过调控上皮间质转化过程参与胰腺癌的侵袭和转移.
목적 탐토RGC-32화E-cadherin단백재이선암조직중적표체,분석기림상병이학의의이급량충단백표체지간적상관성.방법 채용면역조직화학SP법검측42례이선암、12례만성이선염화8례정상이선조직중RGC-32화E-cadherin단백적표체.결과 RGC-32주요표체우이선선포세포포질내；E-cadherin주요표체우정상이선화만성이선염조직이선선포세포포막,이재이선암중칙가출현포질표체화(혹)표체감약등이상표체.이선암조직중RGC-32적양성표체솔이급E-cadherin이상표체솔분별위78.6％( 33/42)화54.8％ (23/42),균현저고우정상이선조직적37.5％ (3/8)화0이급만성이선염조직41.7％(5/12)화8.3％(1/12)(P치균＜0.05).이선암조직RGC-32표체여종류림파결전이화TNM분기유관(P치분별위0.016、0.025),이여환자적년령、성별급종류분화정도무관(P치분별위0.831、1.000、0.629)；E-cadherin이상표체여이선암분화정도、림파결전이화TNM분기유관(P치분별위0.024、0.004、0.004),이여환자년령、성별무관(P치분별위0.970、1.000).RGC-32적양성표체솔여E-cadherin이상표체솔정정상관(r=0.458,P＜0.01).결론 이선암조직중RGC-32양성표체솔급E-cadherin이상표체솔균현저승고,RGC-32가능통과조공상피간질전화과정삼여이선암적침습화전이.
Objective To investigate the expressions of RGC-32 and E-cadherin in pancreatic cancer and analyze their clinicopathological significance and the correlation with each other.Methods SP immunohistochemistry was used to detect the expressions of RGC-32 and E-cadherin in 42 cases of pancreatic cancer tissues,12 cases of chronic pancreatitis tissues and 8 cases of normal pancreatic tissues.Results The positive staining for RGC-32 was predominantly observed in the cytoplasm of pancreatic acinar cells.The positive staining for E-cadherin was mainly observed in the cytomembrane of normal pancreatic and chronic pancreatitis acinar cells,but aberrant expression ( cytoplasm expression and ( or ) weaker expression) could be found in pancreatic cancer cells.The positive expression rate of RGC-32 and aberrant expression rate of E-cadherin were 78.6％ (33/42) and 54.8％ (23/42),respectively,in pancreatic cancer tissues,which were significantly higher than those in normal pancreatic tissues [37.5％ (3/8) and 0] and chronic pancreatitis [41.7％ (5/12)and 8.3％ (1/12) with statisticai significance,P ＜0.05].The expression of RG C-32 in pancreatic cancer was associated with lymph node metastasis and TNM staging (P =0.016,0.025,respectively),but not with age,gender and differentiation degree ( P =0.831,1.000,0.629,respectively).The aberrant expression of E-cadherin was associated with differentiation degree,lymph node metastasis and TNM staging ( P =0.024,0.004,0.004,respectively),but not with age and gender ( P =0.970,1.000,respectively).A significantly positive correlation was found between positive expression rate of RGC-32 and aberrant expression rate of E-cadherin (r =0.458,P ＜0.01 ).Conclusions Both positive expression rate of RGC-32 and aberrant expression rate of E-cadherin are up-regulated significantly in pancreatic cancer tissues and RGC-32 may be involved in the invasion and metastasis of pancreatic cancer by regulating epithelial mesenchymal transition.