中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
4期
478-480
,共3页
熊枝繁%李杰%曹仕琼%刘浩%赵志辉%李丽%李正英%吴燕茹%熊莉娜
熊枝繁%李傑%曹仕瓊%劉浩%趙誌輝%李麗%李正英%吳燕茹%熊莉娜
웅지번%리걸%조사경%류호%조지휘%리려%리정영%오연여%웅리나
肠肿瘤%明胶酶 A%抗原,CD
腸腫瘤%明膠酶 A%抗原,CD
장종류%명효매 A%항원,CD
Intestinal neoplasms%Gelatinase A%Antigens,CD
目的 检测细胞外基质金属蛋白酶诱导因子(EMMPRIN,又称CD147)和基质金属蛋白酶-2(MMP-2)在大肠癌血清中的水平,探讨其与大肠癌恶性程度、侵袭和转移的关系及其相互联系.方法 应用酶联免疫吸附实验(ELISA)方法 测定44例大肠癌、28例大肠息肉和36例健康对照者血清中CD147和MMP-2的水平.结果 大肠癌患者组血清CD147(103.59±40.74)pg/ml和MMP-2(14.92±2.02)ng/ml水平明显高于大肠息肉组(66.27±16.91;10.96±1.71)和健康对照组(63.84±18.71;10.49±1.61)(P＜0.05),差异有统计学意义;大肠癌患者中伴转移组血清中CD147水平显著高于无转移组(P＜0.05),大肠癌患者低分化组血清中MMP-2水平显著高于高,中分化组(P＜0.05),大肠癌患者根治性手术组术后1个月CD147和MMP-2水平明显下降(P＜0.05),而姑息性手术组虽有下降但不显著(P＞0.05).大肠癌患者血清CD147和MMP-2水平呈正相关(r=0.61,P＜0.05).结论 大肠癌患者血清CD147和MMP-2水平增高,与肿瘤的组织分化程度、转移、分期有关,动态观察血清CD147和MMP-2水平的变化可作为大肠癌的诊断、手术方法 的选择、疗效评价及预后监测有意义的参考指标.
目的 檢測細胞外基質金屬蛋白酶誘導因子(EMMPRIN,又稱CD147)和基質金屬蛋白酶-2(MMP-2)在大腸癌血清中的水平,探討其與大腸癌噁性程度、侵襲和轉移的關繫及其相互聯繫.方法 應用酶聯免疫吸附實驗(ELISA)方法 測定44例大腸癌、28例大腸息肉和36例健康對照者血清中CD147和MMP-2的水平.結果 大腸癌患者組血清CD147(103.59±40.74)pg/ml和MMP-2(14.92±2.02)ng/ml水平明顯高于大腸息肉組(66.27±16.91;10.96±1.71)和健康對照組(63.84±18.71;10.49±1.61)(P＜0.05),差異有統計學意義;大腸癌患者中伴轉移組血清中CD147水平顯著高于無轉移組(P＜0.05),大腸癌患者低分化組血清中MMP-2水平顯著高于高,中分化組(P＜0.05),大腸癌患者根治性手術組術後1箇月CD147和MMP-2水平明顯下降(P＜0.05),而姑息性手術組雖有下降但不顯著(P＞0.05).大腸癌患者血清CD147和MMP-2水平呈正相關(r=0.61,P＜0.05).結論 大腸癌患者血清CD147和MMP-2水平增高,與腫瘤的組織分化程度、轉移、分期有關,動態觀察血清CD147和MMP-2水平的變化可作為大腸癌的診斷、手術方法 的選擇、療效評價及預後鑑測有意義的參攷指標.
목적 검측세포외기질금속단백매유도인자(EMMPRIN,우칭CD147)화기질금속단백매-2(MMP-2)재대장암혈청중적수평,탐토기여대장암악성정도、침습화전이적관계급기상호련계.방법 응용매련면역흡부실험(ELISA)방법 측정44례대장암、28례대장식육화36례건강대조자혈청중CD147화MMP-2적수평.결과 대장암환자조혈청CD147(103.59±40.74)pg/ml화MMP-2(14.92±2.02)ng/ml수평명현고우대장식육조(66.27±16.91;10.96±1.71)화건강대조조(63.84±18.71;10.49±1.61)(P＜0.05),차이유통계학의의;대장암환자중반전이조혈청중CD147수평현저고우무전이조(P＜0.05),대장암환자저분화조혈청중MMP-2수평현저고우고,중분화조(P＜0.05),대장암환자근치성수술조술후1개월CD147화MMP-2수평명현하강(P＜0.05),이고식성수술조수유하강단불현저(P＞0.05).대장암환자혈청CD147화MMP-2수평정정상관(r=0.61,P＜0.05).결론 대장암환자혈청CD147화MMP-2수평증고,여종류적조직분화정도、전이、분기유관,동태관찰혈청CD147화MMP-2수평적변화가작위대장암적진단、수술방법 적선택、료효평개급예후감측유의의적삼고지표.
Objective To investigate the serum levels of CD147 and MMP-2 and the relationship between these proteins and colorectal tumor differentiation, Dukes stage. Methods Serum levels of CD147 and MMP-2 in 44 patients with colorectal carcinoma,28 polyp intestinal patients and 36 normal subjects were measured by ELISA. Results Serum levels of CD147(103.59±40.74pg/ml)and MMP-2(14.92±2.02ng/ml)in the cancer patients were significantly higher than those of the polyp intestinal(66.27±16.91;10:96±1.71)and normal control(63.84±18.71;10.49±1.61)(P＜0.05).The levels were associated with tumor differentiation and Dukes stage. Patients with poor differentiation had significantly higher levels of MMP-2 than those of patients with well differentiation. And Dukes C and D stage tumors had significantly higher levels of CD147 than Dukes A and B stage tumors(P＜0.05).The levels of CD147 and MMP-2 declined remarkably after one month of radical operation. Conversely, it decreased illegibly after palliative operation. The serum levels of CD147were positively correlated with MMP-2 in patients with colorectal cancer. Conclusion Elevated CD147 and MMP-2 serum levels are associated with tumor differentiation, invasion, metastasis. Dynamic alterations of serum CD147 and MMP-2 may be used as the indicators of diagnosis, the choice of operative method and the judgment of prognosis in patients with colorectal cancer.
