CAJ | 학술논문

目的对纯钛表面钝化态氧化膜进行生物化学活化改性,探讨生物化学改性对钛表面成骨细胞黏附的影响.方法 24个纯钛试件打磨抛光后均分为4组,每组6个;纯钛组不处理,碱-热处理组行碱-热处理,溶胶涂层组行碱-热处理+溶胶涂层处理,黏附肽组行碱-热处理+溶胶涂层+黏附精氨酸-甘氨酸-天冬氨酸-丝氨酸肽(Glycine-Tyrosine-Arginine-Glycine-Asparticacid-Serine,GYRGDS)处理.使用X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)、傅里叶变换红外光谱计(Fourier transform infrared spectrometer,FTIR)分析各组钛表面化学元素及功能基团组成;在各组钛试件表面接种成骨细胞,经2.05 Pa流体剪切力作用30 min、1 h、2 h,计算细胞残留率.结果碱-热处理组钛表面富含大量活性羟基,溶胶涂层组钛表面富含大量羟氧基团.黏附肽组钛表面经XPS及FTIR分析检测到酰胺峰,显示GYRGDS肽成功固定于钛表面.流体剪切力作用30 min、1 h和2 h后黏附肽组钛表面的成骨细胞残留率(分别为93.0%、54.4%、34.4%)均高于相应时间点其他组.结论 GYRGDS肽修饰后的钛表面成骨细胞黏附稳定性均优于非GYRGDS肽修饰组.
목적 대순태표면둔화태양화막진행생물화학활화개성,탐토생물화학개성대태표면성골세포점부적영향.방법 24개순태시건타마포광후균분위4조,매조6개;순태조불처리,감-열처리조행감-열처리,용효도층조행감-열처리+용효도층처리,점부태조행감-열처리+용효도층+점부정안산-감안산-천동안산-사안산태(Glycine-Tyrosine-Arginine-Glycine-Asparticacid-Serine,GYRGDS)처리.사용X사선광전자능보(X-ray photoelectron spectroscopy,XPS)、부리협변환홍외광보계(Fourier transform infrared spectrometer,FTIR)분석각조태표면화학원소급공능기단조성;재각조태시건표면접충성골세포,경2.05 Pa류체전절력작용30 min、1 h、2 h,계산세포잔류솔.결과 감-열처리조태표면부함대량활성간기,용효도층조태표면부함대량간양기단.점부태조태표면경XPS급FTIR분석검측도선알봉,현시GYRGDS태성공고정우태표면.류체전절력작용30 min、1 h화2 h후점부태조태표면적성골세포잔류솔(분별위93.0%、54.4%、34.4%)균고우상응시간점기타조.결론 GYRGDS태수식후적태표면성골세포점부은정성균우우비GYRGDS태수식조.
Objective To investigate the long-term integrity and the biological function of interface between the bioadhesive peptide modified implant surface and peri-implant tissue. Methods A short bioadhesive peptide containing Glyeine-Tyrosine-Arginine-Glycine-Asparticacid-Serine(GYRGDS) sequence was immobilized onto the titanium implant surface by means of sol-gel coating technique and self-assembled monolayers(SAM). The chemical composition and organic functional groups on the titanium surfaces were characterized using XPS (X-ray photoelectron spectroscopy ) and FTIR (Fourier transform infrared spectrometer). The adhesive strength and stability of osteoblasts on various implant surfaces were compared under flow condition. Results The results showed that alkali/hot water aging treatment could apparently improve the content of -OH functional groups of titanium surface. The chemical reactive Ti-O-Ti bonding at the surface of titanium played a vital role in inducing the formation of organosilane SAM. GYRGDS peptide can be covalently grafted onto the surface of titanium by SAM technique. The resistance of freshly adherent osteoblasts to detachment by flow was shear time dependent. When the four groups were compared under the same flow stress condition(2.05 Pa) at three specific time spans(30 min, 1 h, 2 h), the cells retention rates in GYRGDS-grafted groups were 93.0%, 54. 4%, 34. 4% respectively and were much higher than those in non-coated groups. Conclusions It was suggested that GYRGDS might have positive effects on maintaining stability and adherence of cells onto the substrates under flow condition.