国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2012年
5期
254-257
,共4页
王鑫%冯金炜%王彤%李淼%高志光
王鑫%馮金煒%王彤%李淼%高誌光
왕흠%풍금위%왕동%리묘%고지광
细胞因子%白细胞介素-6%白细胞介素-8%白细胞介素-10%变应性鼻炎
細胞因子%白細胞介素-6%白細胞介素-8%白細胞介素-10%變應性鼻炎
세포인자%백세포개소-6%백세포개소-8%백세포개소-10%변응성비염
Cytokine%IL-6%IL-8%IL-10%Allergic rhinitis
目的 探讨白细胞介素-6、8、10与变应性鼻炎( allergic rhinitis,AR)发病的相关性,为指导临床治疗提供实验依据.方法 应用酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)方法检测35例正常健康对照组以及60例变应性鼻炎患者脱敏治疗前后血清标本中IL-6、IL-8、IL-10的表达水平,并应用SPSS 13.0软件分析治疗前后各指标的表达差异,以及三者之间的相关性.结果 IL-6、IL-8在AR患者血清中高表达,与健康对照组相比差异具有显著性(t=15.213,P＜0.01;t=12.231,P＜0.01),脱敏治疗后表达均下降,治疗前后差异具有统计学意义(t=21.995,P＜0.01;t =19.766,P＜0.01);IL-10在患者血清中表达低于对照组(t=7.446,P＜0.01),脱敏治疗后其表达上升,与治疗前相比差异显著(t=10.228,P＜0.01);IL-6、IL-8在AR患者中的表达呈正相关(r=0.523,P＜0.01),而二者与IL-10的表达呈负相关(r=-0.482,P＜0.01).结论 IL-6、IL-8及IL-10与变应性鼻炎发病过程密切相关,相应的细胞因子或拮抗剂将可能成为候选的治疗药物.
目的 探討白細胞介素-6、8、10與變應性鼻炎( allergic rhinitis,AR)髮病的相關性,為指導臨床治療提供實驗依據.方法 應用酶聯免疫吸附測定(enzyme linked immunosorbent assay,ELISA)方法檢測35例正常健康對照組以及60例變應性鼻炎患者脫敏治療前後血清標本中IL-6、IL-8、IL-10的錶達水平,併應用SPSS 13.0軟件分析治療前後各指標的錶達差異,以及三者之間的相關性.結果 IL-6、IL-8在AR患者血清中高錶達,與健康對照組相比差異具有顯著性(t=15.213,P＜0.01;t=12.231,P＜0.01),脫敏治療後錶達均下降,治療前後差異具有統計學意義(t=21.995,P＜0.01;t =19.766,P＜0.01);IL-10在患者血清中錶達低于對照組(t=7.446,P＜0.01),脫敏治療後其錶達上升,與治療前相比差異顯著(t=10.228,P＜0.01);IL-6、IL-8在AR患者中的錶達呈正相關(r=0.523,P＜0.01),而二者與IL-10的錶達呈負相關(r=-0.482,P＜0.01).結論 IL-6、IL-8及IL-10與變應性鼻炎髮病過程密切相關,相應的細胞因子或拮抗劑將可能成為候選的治療藥物.
목적 탐토백세포개소-6、8、10여변응성비염( allergic rhinitis,AR)발병적상관성,위지도림상치료제공실험의거.방법 응용매련면역흡부측정(enzyme linked immunosorbent assay,ELISA)방법검측35례정상건강대조조이급60례변응성비염환자탈민치료전후혈청표본중IL-6、IL-8、IL-10적표체수평,병응용SPSS 13.0연건분석치료전후각지표적표체차이,이급삼자지간적상관성.결과 IL-6、IL-8재AR환자혈청중고표체,여건강대조조상비차이구유현저성(t=15.213,P＜0.01;t=12.231,P＜0.01),탈민치료후표체균하강,치료전후차이구유통계학의의(t=21.995,P＜0.01;t =19.766,P＜0.01);IL-10재환자혈청중표체저우대조조(t=7.446,P＜0.01),탈민치료후기표체상승,여치료전상비차이현저(t=10.228,P＜0.01);IL-6、IL-8재AR환자중적표체정정상관(r=0.523,P＜0.01),이이자여IL-10적표체정부상관(r=-0.482,P＜0.01).결론 IL-6、IL-8급IL-10여변응성비염발병과정밀절상관,상응적세포인자혹길항제장가능성위후선적치료약물.
Objective To provide experimental evidence and guide clinical treatment by exploring the role of IL-6,IL-8,IL-10 in allergic rhinitis patients.Methods The serum samples were collected from 35 cases of normal healthy controls and 60 patients with allergic rhinitis line before and after the desensitization treatment.The serum expression levels of IL-6,IL-8,and IL-10 were detected by double antibody sandwich ELISA method.SPSS 13.0 software was used for statistical analysis.Results IL-6,IL-8 in the AR patients were with high expression compared with the control group ( t =15.213,P ＜0.01 ; t =12.231,P ＜ 0.01 ) after desensitization treatment,the expression decreased compared with the pre-treatment and there were high significant difference ( t =21.995,P ＜ 0.01 ; t =19.766,P ＜ 0.01 ) ; IL- 10 expression in the serum of patients was with lower than the control group,the difference was significant (t =7.446,P ＜ 0.01 ).With desensitization therapy,its expression was rose compared with the pre-treatment and significant difference was found ( t =10.228,P ＜ 0.01 ).Besides,IL-6 and IL-8 expressions in AR patients showed a positive correlation ( r =0.523,P ＜ 0.01 )and negative correlation with IL-10 ( r =- 0.482,P ＜ 0.01 ).Conclusion IL-6,IL-8,and IL-10 play an important role in the pathogenesis of allergic rhinitis.The corresponding cytokines or antagonists will likely become the candidate therapeutic drugs.
