中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2011年
10期
621-624
,共4页
韩会民%郭琳%姜丽娟%蒋晓宇%吕雅丽%庞金奎%白忠民%车蔚娟%徐荣慧%于萍%李强
韓會民%郭琳%薑麗娟%蔣曉宇%呂雅麗%龐金奎%白忠民%車蔚娟%徐榮慧%于萍%李彊
한회민%곽림%강려연%장효우%려아려%방금규%백충민%차위연%서영혜%우평%리강
中药%解毒生肌膏%糖尿病足%基因芯片%大鼠
中藥%解毒生肌膏%糖尿病足%基因芯片%大鼠
중약%해독생기고%당뇨병족%기인심편%대서
Herb%Antidotal and Myogenic Ointment%Diabetes foot ulcer%cDNA microarray%Rats
目的 采用基因芯片技术探讨中药解毒生肌膏对大鼠糖尿病足溃疡的作用机制.方法 腹腔注射链脲佐菌素复制糖尿病大鼠模型,喂养到6个月时结扎腘动脉下端并切断,7个月时造成足底部皮肤深Ⅱ度烫伤复制大鼠糖尿病足溃疡模型.将模型大鼠按随机数字表法分为中药组、表皮生长因子组、模型对照组,每组15只.烫伤后分别给予中药解毒生肌膏、重组人表皮生长因子、生理盐水局部外用,每2d换药1次,治疗30 d后观察大鼠治疗前后溃疡面积及愈合情况.提取各组溃疡区组织总RNA,应用大鼠细胞因子芯片进行组间基因比较.结果 与模型对照组比较,表皮生长因子组有25个基因发生改变,其中上调23个,下调2个；中药组有30个基因发生改变,其中上调29个,下调1个.与表皮生长因子组比较,中药组有16个基因发生改变,其中上调11个,下调5个.这些基因涉及神经营养因子、趋化因子.结论 中药解毒生肌膏和表皮生长因子能够通过影响各种炎症反应过程中的细胞因子平衡,促进糖尿病足溃疡的愈合,其中以中药解毒生肌膏的效果更好.
目的 採用基因芯片技術探討中藥解毒生肌膏對大鼠糖尿病足潰瘍的作用機製.方法 腹腔註射鏈脲佐菌素複製糖尿病大鼠模型,餵養到6箇月時結扎腘動脈下耑併切斷,7箇月時造成足底部皮膚深Ⅱ度燙傷複製大鼠糖尿病足潰瘍模型.將模型大鼠按隨機數字錶法分為中藥組、錶皮生長因子組、模型對照組,每組15隻.燙傷後分彆給予中藥解毒生肌膏、重組人錶皮生長因子、生理鹽水跼部外用,每2d換藥1次,治療30 d後觀察大鼠治療前後潰瘍麵積及愈閤情況.提取各組潰瘍區組織總RNA,應用大鼠細胞因子芯片進行組間基因比較.結果 與模型對照組比較,錶皮生長因子組有25箇基因髮生改變,其中上調23箇,下調2箇；中藥組有30箇基因髮生改變,其中上調29箇,下調1箇.與錶皮生長因子組比較,中藥組有16箇基因髮生改變,其中上調11箇,下調5箇.這些基因涉及神經營養因子、趨化因子.結論 中藥解毒生肌膏和錶皮生長因子能夠通過影響各種炎癥反應過程中的細胞因子平衡,促進糖尿病足潰瘍的愈閤,其中以中藥解毒生肌膏的效果更好.
목적 채용기인심편기술탐토중약해독생기고대대서당뇨병족궤양적작용궤제.방법 복강주사련뇨좌균소복제당뇨병대서모형,위양도6개월시결찰객동맥하단병절단,7개월시조성족저부피부심Ⅱ도탕상복제대서당뇨병족궤양모형.장모형대서안수궤수자표법분위중약조、표피생장인자조、모형대조조,매조15지.탕상후분별급여중약해독생기고、중조인표피생장인자、생리염수국부외용,매2d환약1차,치료30 d후관찰대서치료전후궤양면적급유합정황.제취각조궤양구조직총RNA,응용대서세포인자심편진행조간기인비교.결과 여모형대조조비교,표피생장인자조유25개기인발생개변,기중상조23개,하조2개；중약조유30개기인발생개변,기중상조29개,하조1개.여표피생장인자조비교,중약조유16개기인발생개변,기중상조11개,하조5개.저사기인섭급신경영양인자、추화인자.결론 중약해독생기고화표피생장인자능구통과영향각충염증반응과정중적세포인자평형,촉진당뇨병족궤양적유합,기중이중약해독생기고적효과경호.
Objective To investigate the underlying mechanism for the therapeutic effect of a traditional Chinese medicinal recipe,Antidotal and Myogenic Ointment(解毒生肌膏,AMO),on the foot ulcer in diabetic rat using cDNA microarray technology.Methods 45 rats were made diabetic by i.p.injection of streptozocin.The treated animals were then fed for 6 months,and subjected to the dissection of distal popliteal artery after ligation of the vessels.Another month later,grade Ⅱ burn injury was produced on the bottom of their foot as a model of diabetic foot ulcer.The rats were then randomly divided into three groups (15 each) to receive AMO,epidermal growth factor (EGF) and saline for 30 days,with dressing change in every 2 days.The area of ulcer wound and their healing rate were recorded before and after the treatment.Total RNA was extracted from the tissue samples collected near the wound,and the expression profile of cytokine genes demonstrated using the microarry for rats.Results In comparison with the saline group difference in the level of expression was found in 25 genes (23 0f them were up-regulated and 2 down-regulated) in EGF group,and 30 genes in AMO groups (29 0f them up-regulated and l down-regulated).In comparison with EGF group,difference in level of expression was found in 16 genes in AMO group,with 11 up-regulated and 5 down-regulated.Neurotrophic factors and chemotactic factors,etc were among the genes involved.Conclusion In comparison with EGF,AMO is more effective in the treatment of foot ulcer in diabetic rats.It is possible that AMO produces such effects through the regulation of balance in cytokine expression.
