中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
3期
372-375
,共4页
李戈辉%李李%沈金美%裴万敏
李戈輝%李李%瀋金美%裴萬敏
리과휘%리리%침금미%배만민
再灌注损伤%肺%Clara细胞%炎症
再灌註損傷%肺%Clara細胞%炎癥
재관주손상%폐%Clara세포%염증
Reporfusion injury%Lung%Clara cell%Inflammatory reaction
目的 探讨Clara细胞在兔肺缺血再灌注(IR)损伤中的作用.方法 健康家兔24只.随机分为3组(n=8):假手术组(S组)、IR组、肺Clara细胞剥除+IR组(CIR组).CIR组置于萘气体浓度为89.28 mg/m3的气体箱中12 h,S组和IR组置于无萘气体箱中12 h.取出正常饲养24 h后.IR组和CIR组采用阻断左肺门60 min,再灌注120 min的方法制备肺IR模型,S组不予阻断肺门.于再灌注120 min时采集颈动脉血样5 ml,测定血清TNF-α和总蛋白浓度,收集支气管肺泡灌洗液(BALF),计数WBC、中性粒细胞(PMN),计算PMN百分比,取肺组织测定MDA含量和湿/干重比值(W/D比值),观察病理学改变和Clara细胞的分布情况.结果 S组肺泡完整,肺实质无炎性细胞浸润,可见较多的Clara细胞;IR组肺不张伴肺气肿,肺间质增宽水肿,中度炎性细胞浸润,肺泡腔内有淡黄色渗出,渗出物有WBC,亦可见较多的Clara细胞;CIR组炎性细胞浸润严重,肺泡腔内渗出物多.WBC较多,血管腔变窄,甚至出现无复流现象,Clara细胞少见.与S组比较,IR组和CIR组BALF中WBC计数、PMN百分比、血清TNF-α浓度、肺组织MDA含量及W/D比值升高(P<0.05 );与IR组比较,CIR组BALF中WBC计数、PMN百分比、血清TNF-α浓度、肺组织MDA含量及W/D比值升高(P＜0.05).结论 肺IR损伤时兔肺内Clara细胞通过抑制炎性反应产生内源性的保护作用.
目的 探討Clara細胞在兔肺缺血再灌註(IR)損傷中的作用.方法 健康傢兔24隻.隨機分為3組(n=8):假手術組(S組)、IR組、肺Clara細胞剝除+IR組(CIR組).CIR組置于萘氣體濃度為89.28 mg/m3的氣體箱中12 h,S組和IR組置于無萘氣體箱中12 h.取齣正常飼養24 h後.IR組和CIR組採用阻斷左肺門60 min,再灌註120 min的方法製備肺IR模型,S組不予阻斷肺門.于再灌註120 min時採集頸動脈血樣5 ml,測定血清TNF-α和總蛋白濃度,收集支氣管肺泡灌洗液(BALF),計數WBC、中性粒細胞(PMN),計算PMN百分比,取肺組織測定MDA含量和濕/榦重比值(W/D比值),觀察病理學改變和Clara細胞的分佈情況.結果 S組肺泡完整,肺實質無炎性細胞浸潤,可見較多的Clara細胞;IR組肺不張伴肺氣腫,肺間質增寬水腫,中度炎性細胞浸潤,肺泡腔內有淡黃色滲齣,滲齣物有WBC,亦可見較多的Clara細胞;CIR組炎性細胞浸潤嚴重,肺泡腔內滲齣物多.WBC較多,血管腔變窄,甚至齣現無複流現象,Clara細胞少見.與S組比較,IR組和CIR組BALF中WBC計數、PMN百分比、血清TNF-α濃度、肺組織MDA含量及W/D比值升高(P<0.05 );與IR組比較,CIR組BALF中WBC計數、PMN百分比、血清TNF-α濃度、肺組織MDA含量及W/D比值升高(P＜0.05).結論 肺IR損傷時兔肺內Clara細胞通過抑製炎性反應產生內源性的保護作用.
목적 탐토Clara세포재토폐결혈재관주(IR)손상중적작용.방법 건강가토24지.수궤분위3조(n=8):가수술조(S조)、IR조、폐Clara세포박제+IR조(CIR조).CIR조치우내기체농도위89.28 mg/m3적기체상중12 h,S조화IR조치우무내기체상중12 h.취출정상사양24 h후.IR조화CIR조채용조단좌폐문60 min,재관주120 min적방법제비폐IR모형,S조불여조단폐문.우재관주120 min시채집경동맥혈양5 ml,측정혈청TNF-α화총단백농도,수집지기관폐포관세액(BALF),계수WBC、중성립세포(PMN),계산PMN백분비,취폐조직측정MDA함량화습/간중비치(W/D비치),관찰병이학개변화Clara세포적분포정황.결과 S조폐포완정,폐실질무염성세포침윤,가견교다적Clara세포;IR조폐불장반폐기종,폐간질증관수종,중도염성세포침윤,폐포강내유담황색삼출,삼출물유WBC,역가견교다적Clara세포;CIR조염성세포침윤엄중,폐포강내삼출물다.WBC교다,혈관강변착,심지출현무복류현상,Clara세포소견.여S조비교,IR조화CIR조BALF중WBC계수、PMN백분비、혈청TNF-α농도、폐조직MDA함량급W/D비치승고(P<0.05 );여IR조비교,CIR조BALF중WBC계수、PMN백분비、혈청TNF-α농도、폐조직MDA함량급W/D비치승고(P＜0.05).결론 폐IR손상시토폐내Clara세포통과억제염성반응산생내원성적보호작용.
Objective To investigate the role of Clara cells in lung ischemia-reperfusion (I/R) injury in rabbits.Methods Twenty-four healthy 10-12 month old rabbits of either sex weighing 1.5-2.0 kg were randomly divided into 3 groups ( n = 8 each) ; group A sham operation (group S) ; group B lung I/R and group C Clara cell elimination+ lung I/R. The animals were anesthetized with iv pentobarbital 30 mg/kg , tracheostomized and mechanically ventilated. In group B and C lung I/R was induced by clamping the left hilum of lung for 60 min followed by 120 min repeffusion. In group C Clara ceils were eliminated by ventilating the lungs with 89.28 mg/m2 naphthalin vapor for 12 h before lung I/R. The animals were killed by iv KCI at the end of 120 min reperfusion after lung isehemia. The left lung was immediately removed for microscopic examination, determination of W/D lung weight ratio and serum TNF-α level and MDA content. The percentage of neutrophi] in bronchoalveolar lavage fluid (BALF) was detected as index of lung injury. The expression of Clara cell secreting protein (CCSP) in the lung was detected by immuno-histoebemistry to indicate the number and distribution of Clara cells in the lung.Results Microscopic examination showed that there were severe leukocyte infiltration in alveolar spaces, alveolar edema and destroyed alveolar structure in group B and C. The serum TNF-u leve],W/D ratio and MDA content in the left lung and neutrophil percentage and WBC counts in BALF were significantly higher in group C than in group B. Conclusion Clara cells can protect the lungs against I/R injury through inhibiting inflammatory responses.
