CAJ | 학술논문

目的探讨血管内介入方法制作偏侧帕金森病(PD)猴模型的稳定技术和方法,评价其有效性及实用性.方法 4只健康老年恒河猴,经右股动脉插管至颈内动脉注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP),剂量为1.0 ～ 1.2 mg/kg,药物浓度为0.2g/L.术后2周进行运动功能评分,行阿扑吗啡诱转试验,10周后采用免疫组织化学方法检测中脑多巴胺能神经元变化.结果 4只恒河猴制作模型成功.免疫组织化学检测示注药侧中脑黑质酪氨酸羟化酶(TH)阳性细胞形态改变,数量减少,注药侧平均为(20.3±4.7)个,正常侧平均为(66.3±11.9)个(P＜O.05).结论血管内介入方法制作偏侧帕金森病猴模型是一种安全、有效的方法,但对硬件及技术条件有一定要求.
목적 탐토혈관내개입방법제작편측파금삼병(PD)후모형적은정기술화방법,평개기유효성급실용성.방법 4지건강노년항하후,경우고동맥삽관지경내동맥주사1-갑기-4-분기-1,2,3,6-사경필정(MPTP),제량위1.0 ～ 1.2 mg/kg,약물농도위0.2g/L.술후2주진행운동공능평분,행아복마배유전시험,10주후채용면역조직화학방법검측중뇌다파알능신경원변화.결과 4지항하후제작모형성공.면역조직화학검측시주약측중뇌흑질락안산간화매(TH)양성세포형태개변,수량감소,주약측평균위(20.3±4.7)개,정상측평균위(66.3±11.9)개(P＜O.05).결론 혈관내개입방법제작편측파금삼병후모형시일충안전、유효적방법,단대경건급기술조건유일정요구.
Objective To establish the hemiparkinsonism model of rhesus monkey by unilateral internal carotid artery (ICA) infusion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and evaluate the effectiveness and practicality.Methods Four healthy rhesus monkeys aged from 7 to 9 received MPTP injection through ICA by means of endovasctdar operation from the femoral artery.The dose of MPTP was 1.0-1.2 mg/kg,and the drug level was 0.2 mg/mL.Motor function scale was evaluated 2 weeks after surgery.Results All the rhesus monkeys developed PD successfully.The tyrosine hydroxylase (TH)-immunoreactive neurons in the mesencephalon of the injection side were deformed and decreased,displaying significant difference with that in control side ( 20.3 ± 4.7 vs 66.3 ± 11.9,P ＜ 0.05 ).Conclusion The rhesus monkey model of hemiparkinsonism could be induced by unilateral ICA infusion of MPTP and endovascular operation was safe and effective.