CAJ | 학술논문

目的观察神经生长液含药血清对体外培养的背根神经节神经元生长和雪旺细胞增殖的影响,探讨其促损伤神经修复的机制.方法制备神经生长含药血清;采用新生大鼠背根神经节及雪旺细胞体外培养,通过形态学分析,观察神经生长液含药血清对背根神经节神经元突起生长的影响;采用MTr、BrdU标记和细胞计数法,观察神经生长液含药血清对雪旺细胞活力及其增殖的影响.结果高剂量的神经生长液含药血清,可有效地促进大鼠背根神经节神经元生长;高、低剂量的神经生长液含药血清均能显著增加大鼠雪旺细胞的活力,促进雪旺细胞增殖.结论神经生长液含药血清具有促进神经元生长、增强雪旺细胞活力、促进雪旺细胞增殖作用.
목적 관찰신경생장액함약혈청대체외배양적배근신경절신경원생장화설왕세포증식적영향,탐토기촉손상신경수복적궤제.방법 제비신경생장함약혈청;채용신생대서배근신경절급설왕세포체외배양,통과형태학분석,관찰신경생장액함약혈청대배근신경절신경원돌기생장적영향;채용MTr、BrdU표기화세포계수법,관찰신경생장액함약혈청대설왕세포활력급기증식적영향.결과 고제량적신경생장액함약혈청,가유효지촉진대서배근신경절신경원생장;고、저제량적신경생장액함약혈청균능현저증가대서설왕세포적활력,촉진설왕세포증식.결론 신경생장액함약혈청구유촉진신경원생장、증강설왕세포활력、촉진설왕세포증식작용.
Objective To investigate the effects of blood serum of nerve growth granule (NGG) on proliferation of Schwann cells. Methods Blood serum of NGG and methycobal were prepared. Schwann cells and dorsal root ganglia (DRG) of neonate rats were cultured in vitro under various conditions as following: high and low dose of NGG groups, methycobal group (positive control) and blood serum of rat abdominal aorta (negative control) group. MTr, BrdU marking method and cytometry were conducted to explore the effects of blood serum of NGG on activity and proliferation of Schwann cells. DRG neurite outgrowth was also observed. Results High and low dose of NGG groups could remarkably enhance Schwann cell activity and promote proliferation of Schwann cells. Conclusion Blood serum of NGG could enhance the activity and proliferation of Schwann cells.