CAJ | 학술논문

目的探讨分化抑制因子1(ID1)在全反式维甲酸(ATRA)诱导急性早幼粒细胞白血病(APL)细胞分化中的作用.方法运用基因芯片、放线菌酮抑制试验、实时定量RT-PCR和Western blot.方法检测ID1存ATRA诱导的APL细胞系及原代APL细胞分化过程中表达情况.结果在ATRA污导的NB4细胞和APL初诊患者原代细胞分化过程巾ID1基因的表达上调,ATRA诱导NB4细胞2hID1表达达高峰,其相对表达水平与对照相比升高359.4±48.7倍;ATRA处理APL患者骨髓细胞ID1表达水平2 h达高峰,其中1例患者晕复3次检测的.结果为对照的(311.1±48.7)倍.而且ID1基因的上调不需要其他蛋白的合成.而在ATRA处理ATRA耐药细胞系NB4-R2细胞未见ID1的表达发生明显变化.结论 ID1基因可能作为ATRA的靶基因参与了ATRA诱导的粒系分化.
목적 탐토분화억제인자1(ID1)재전반식유갑산(ATRA)유도급성조유립세포백혈병(APL)세포분화중적작용.방법 운용기인심편、방선균동억제시험、실시정량RT-PCR화Western blot.방법 검측ID1존ATRA유도적APL세포계급원대APL세포분화과정중표체정황.결과 재ATRA오도적NB4세포화APL초진환자원대세포분화과정건ID1기인적표체상조,ATRA유도NB4세포2hID1표체체고봉,기상대표체수평여대조상비승고359.4±48.7배;ATRA처리APL환자골수세포ID1표체수평2 h체고봉,기중1례환자훈복3차검측적.결과 위대조적(311.1±48.7)배.이차ID1기인적상조불수요기타단백적합성.이재ATRA처리ATRA내약세포계NB4-R2세포미견ID1적표체발생명현변화.결론 ID1기인가능작위ATRA적파기인삼여료ATRA유도적립계분화.
Objective To study the role of inhibitor of differentiation l(Idl)in ATRA-induced a-cute promyelocytic leukemia(APL)cells differentiation.Methods The expression of Idl was detected by cDNA microarray.Cycloheximide inhibition test.Real-time RT-PCR and western blot.Results The expres-sion of Idl gene was up-regulated in ATRA-induced NB4 cells and APL cells from two patients and was inde-pendent on other proteins synthesis.Idl expression level reached the peak at 2 h in NB4 cells induced by ATRA.Its relative expression level was(359.4±48.7)-fold greater than contro1.Idl expression level reached the peak at 2 h in bone marrow cells from APL patents treated with ATRA.And itS level detected 3 times in one of the patient was(311.1±48.7)fold of contro1.The expression of Idl protein was not up-reg-ulated in ATRA resistant NB4-R2 cells after ATRA treatment.Conclusion Idl may be involved in ATRA-induced granulocytic difierentiation as an ATRA-targeted gene.