中国狂犬病N基因分子流行病学调查研究
중국광견병N기인분자류행병학조사연구
Molecular epidemiology investigation of rabies virus nucleoprotein genes in China
  • 万方数据
    中华实验和临床病毒学杂志 중화실험화림상병독학잡지
    CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
    2010年 2期 82-84 ,共3页

    李浩%陶晓燕%王晓光%雷永良%张海林%王显军%丁淑军%张红%戴德芳%沈蕊华%唐青

    리호%도효연%왕효광%뢰영량%장해림%왕현군%정숙군%장홍%대덕방%침예화%당청

    狂犬病病毒%遗传学,生物化学%基因%变异(遗传学)%疾病暴发流行 狂犬病病毒%遺傳學,生物化學%基因%變異(遺傳學)%疾病暴髮流行
    광견병병독%유전학,생물화학%기인%변이(유전학)%질병폭발류행
    Rabies virus%Genetics,biochemical%Genes%Variation (genetic)%Disease outbreaks
    目的 通过分析近年中国狂犬病病毒流行株N基因特征和遗传变异状况,探讨我国狂犬病的流行分布与狂犬病病毒遗传变异间的关系.方法 对不同流行地区、不同宿主动物来源的狂犬病标本,进行病毒N基因编码区下游720个核苷酸序列测定、序列同源性比较和种系发生分析.结果 获得相应区段的狂犬病病毒核苷酸序列34份;核苷酸序列同源性87.5%～100%,推导的氨基酸序列同源性93.3%～99.6%,核苷酸序列的变异主要为同义突变.结论 34个病毒标本都属于基因Ⅰ型狂犬病病毒且具有地域性特征.我国狂犬病高发病地区狂犬病病毒来源多样化,且病毒呈现从高发病地区向周边地区的传播和扩散,野生动物与家养动物之间,本国动物与外国动物之间,可能均存在着狂犬病病毒的交叉感染和相互传播.
    목적 통과분석근년중국광견병병독류행주N기인특정화유전변이상황,탐토아국광견병적류행분포여광견병병독유전변이간적관계.방법 대불동류행지구、불동숙주동물래원적광견병표본,진행병독N기인편마구하유720개핵감산서렬측정、서렬동원성비교화충계발생분석.결과 획득상응구단적광견병병독핵감산서렬34빈;핵감산서렬동원성87.5%～100%,추도적안기산서렬동원성93.3%～99.6%,핵감산서렬적변이주요위동의돌변.결론 34개병독표본도속우기인Ⅰ형광견병병독차구유지역성특정.아국광견병고발병지구광견병병독래원다양화,차병독정현종고발병지구향주변지구적전파화확산,야생동물여가양동물지간,본국동물여외국동물지간,가능균존재착광견병병독적교차감염화상호전파.
    Objective To study the relationship between the distribution of rabies virus and genetic variation,the genetic characterization and variation of rabies virus strains in China were analyzed.Methods The downstream 720 nucleotides of Nucleoprotein (N) gene coding region of the rabies specimens from different areas and host animals were sequenced,and then homology and phylogenesis were analyzed.Results Nucleotide similarities of 34 N gene sequences were 87.5%-100%,and the deduced amino acid similarities were 93.3%-99.6%.Most of the nucleotide variations were synonymous mutations.Conclusion The 34 rabies specimens all belong to genotype Ⅰ and are of regional characteristic.The rabies viruses in high-incidence areas in China are of various origins and present the transmission tendency from high-incidence areas to surrounding regions.There may be cross-infection and mutual spread of rabies virus between wildlife and domestic animals as well as native and foreign animals.
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