植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2003年
1期
73-75
,共3页
方晓华%张方%吴乃虎%胡适宜
方曉華%張方%吳迺虎%鬍適宜
방효화%장방%오내호%호괄의
甘薯%质体遗传%DNA指纹图谱%正反交子代
甘藷%質體遺傳%DNA指紋圖譜%正反交子代
감서%질체유전%DNA지문도보%정반교자대
Ipomoea batatas%plastid inheritance%DNA restriction fingerprinting%hybrids of reciprocal cross%Convolvulaceae
用DNA指纹图谱分析了甘薯(Ipomoea batatas Lam.)徐薯18和AB78-1品系及它们的正反交子代叶绿体DNA,结果显示子代叶绿体DNA指纹均与母本相同,而未发现与父本或双亲相同的指纹图谱,因此在该杂交组合中质体遗传方式为母系遗传.这个结论与先前根据细胞学研究所推测的甘薯质体遗传方式不同,表明旋花科植物可能并不存在一个一致的父系或双亲质体传递模式.DNA指纹图谱分析用于质体遗传的研究尚未见报道,本文对其优越性进行了讨论.
用DNA指紋圖譜分析瞭甘藷(Ipomoea batatas Lam.)徐藷18和AB78-1品繫及它們的正反交子代葉綠體DNA,結果顯示子代葉綠體DNA指紋均與母本相同,而未髮現與父本或雙親相同的指紋圖譜,因此在該雜交組閤中質體遺傳方式為母繫遺傳.這箇結論與先前根據細胞學研究所推測的甘藷質體遺傳方式不同,錶明鏇花科植物可能併不存在一箇一緻的父繫或雙親質體傳遞模式.DNA指紋圖譜分析用于質體遺傳的研究尚未見報道,本文對其優越性進行瞭討論.
용DNA지문도보분석료감서(Ipomoea batatas Lam.)서서18화AB78-1품계급타문적정반교자대협록체DNA,결과현시자대협록체DNA지문균여모본상동,이미발현여부본혹쌍친상동적지문도보,인차재해잡교조합중질체유전방식위모계유전.저개결론여선전근거세포학연구소추측적감서질체유전방식불동,표명선화과식물가능병불존재일개일치적부계혹쌍친질체전체모식.DNA지문도보분석용우질체유전적연구상미견보도,본문대기우월성진행료토론.
The inheritance of chloroplast DNA (cpDNA) in sweet potato ( Ipomoea batatas Lam. ) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushul8 and AB78-1 were found to be identical to those of their female parents, which reveals that cpDNA of sweet potato is maternally inherited in this intervarietal crossing. This maternal cpDNA transmission pattern does not accord with the putative one based on former cytological studies. The plastid inheritance in Convolvulaceae has been briefly reviewed in this study, and the utility of DNA restriction fingerprinting analysis in the study of plastid inheritance is also discussed.