时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
7期
1836-1837
,共2页
石斛%rbcL基因%序列分析%系统发育
石斛%rbcL基因%序列分析%繫統髮育
석곡%rbcL기인%서렬분석%계통발육
Dendrobium%rbcL gene%Sequence analysis%Phylogeny
目的 探讨10种石斛植物叶绿体rbcL基因序列变异和系统发育关系.方法 根据Genebank已经公布的植物rbcL基因序列设计一对引物,用于石斛rbcL基因PCR扩增.基因序列排列用Clustal X软件完成,应用Seqnconverter软件分析序列的长度、GC含量、GpC量、GCskew值等;用Mega 4.1软件分析转换值、颠换值、转换/颠换比;采用最大简约法(maximum parsimony method)获得最大简约系统树;应用自展法(bootstrap)检验系统树,自展次数为1 000次.结果 10种石斛植物叶绿体rbcL基因的长度范围为944～950 bp,其中536 bp为保守位点,466 bp为变异位点,439 bp为具有系统发育信息的信息位点.基因中GC含量为40.61%～41.37%,GpC含量为4.03%～4.78%,GCskew 值为0.043 2～0.082 1.序列转换/颠换比(Si/Sv)为0.9.结论 利用PCR和核酸测序方法可以获得rbcL基因的核苷酸序列,能为石斛植物的系统发育研究提供核苷酸序列方面的资料.
目的 探討10種石斛植物葉綠體rbcL基因序列變異和繫統髮育關繫.方法 根據Genebank已經公佈的植物rbcL基因序列設計一對引物,用于石斛rbcL基因PCR擴增.基因序列排列用Clustal X軟件完成,應用Seqnconverter軟件分析序列的長度、GC含量、GpC量、GCskew值等;用Mega 4.1軟件分析轉換值、顛換值、轉換/顛換比;採用最大簡約法(maximum parsimony method)穫得最大簡約繫統樹;應用自展法(bootstrap)檢驗繫統樹,自展次數為1 000次.結果 10種石斛植物葉綠體rbcL基因的長度範圍為944～950 bp,其中536 bp為保守位點,466 bp為變異位點,439 bp為具有繫統髮育信息的信息位點.基因中GC含量為40.61%～41.37%,GpC含量為4.03%～4.78%,GCskew 值為0.043 2～0.082 1.序列轉換/顛換比(Si/Sv)為0.9.結論 利用PCR和覈痠測序方法可以穫得rbcL基因的覈苷痠序列,能為石斛植物的繫統髮育研究提供覈苷痠序列方麵的資料.
목적 탐토10충석곡식물협록체rbcL기인서렬변이화계통발육관계.방법 근거Genebank이경공포적식물rbcL기인서렬설계일대인물,용우석곡rbcL기인PCR확증.기인서렬배렬용Clustal X연건완성,응용Seqnconverter연건분석서렬적장도、GC함량、GpC량、GCskew치등;용Mega 4.1연건분석전환치、전환치、전환/전환비;채용최대간약법(maximum parsimony method)획득최대간약계통수;응용자전법(bootstrap)검험계통수,자전차수위1 000차.결과 10충석곡식물협록체rbcL기인적장도범위위944～950 bp,기중536 bp위보수위점,466 bp위변이위점,439 bp위구유계통발육신식적신식위점.기인중GC함량위40.61%～41.37%,GpC함량위4.03%～4.78%,GCskew 치위0.043 2～0.082 1.서렬전환/전환비(Si/Sv)위0.9.결론 이용PCR화핵산측서방법가이획득rbcL기인적핵감산서렬,능위석곡식물적계통발육연구제공핵감산서렬방면적자료.
Objective To explore the sequence variation of rbcL Gene and Phylogenetic relationship of Dendrobium plants.Methods A pair of primers was designed to amplify the rbcL gene.Gene sequence,the contents of G+C,the contents of GpC,the value of GCskew,transitionsal pairs,transversional pairs and si/sv were conducted.Phylogenetic analysis was conducted using the maximum - parsimony (MP) method.Bootstrap analysis were carried out to evaluate statistical reliability based on 1000 resamplings of the data set.Results 944～950 bp nucleotides were sequenced in the chloroplast rbcL gene from Dendrobium plants.Conserved sites,variable sites and phylogenetically informative sites were 536 bp,466 bp,439 bp respectively.The contents of GC,the contents of GpC,the value of GC skew and transitionsal pairs/transversional pairs were 40.61%～41.37%,4.03%～4.78%,0.043 2～0.082 1 and 0.9 respectively.Conclusion This paper offers DNA sequence data for the study of Dendrobium phylogeny.
