华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
5期
673-676
,共4页
韩高雄%蔡明%王国斌%陶凯雄%蔡昌学
韓高雄%蔡明%王國斌%陶凱雄%蔡昌學
한고웅%채명%왕국빈%도개웅%채창학
Livin%Survivin%RNA干扰%基因治疗
Livin%Survivin%RNA榦擾%基因治療
Livin%Survivin%RNA간우%기인치료
Livin%Survivin%RNA interference%gene therapy
目的 构建Livin和Survivin联合靶向的siRNA重组表达载体,探讨用RNA干扰方法同时下调Livin和Survivin基因表达的增效作用.方法 通过前期的实验,分别从靶向Livin基因和Survivin基因的siRNA序列中各挑选出1对最有效的siRNA序列,然后采用1个载体编码2个shRNA技术,将其构建到同一个真核表达载体上,转化DH5α菌株,提取质粒进行酶切鉴定和测序分析.结果 经酶切鉴定和测序分析证实Livin和Survivin联合靶向的siRNA重组表达载体构建成功.结论 Livin和Survivin联合靶向的siRNA重组表达载体构建成功.
目的 構建Livin和Survivin聯閤靶嚮的siRNA重組錶達載體,探討用RNA榦擾方法同時下調Livin和Survivin基因錶達的增效作用.方法 通過前期的實驗,分彆從靶嚮Livin基因和Survivin基因的siRNA序列中各挑選齣1對最有效的siRNA序列,然後採用1箇載體編碼2箇shRNA技術,將其構建到同一箇真覈錶達載體上,轉化DH5α菌株,提取質粒進行酶切鑒定和測序分析.結果 經酶切鑒定和測序分析證實Livin和Survivin聯閤靶嚮的siRNA重組錶達載體構建成功.結論 Livin和Survivin聯閤靶嚮的siRNA重組錶達載體構建成功.
목적 구건Livin화Survivin연합파향적siRNA중조표체재체,탐토용RNA간우방법동시하조Livin화Survivin기인표체적증효작용.방법 통과전기적실험,분별종파향Livin기인화Survivin기인적siRNA서렬중각도선출1대최유효적siRNA서렬,연후채용1개재체편마2개shRNA기술,장기구건도동일개진핵표체재체상,전화DH5α균주,제취질립진행매절감정화측서분석.결과 경매절감정화측서분석증실Livin화Survivin연합파향적siRNA중조표체재체구건성공.결론 Livin화Survivin연합파향적siRNA중조표체재체구건성공.
Objective To construct a novel siRNA expression vector targeting Livin and Survivin simultaneously,and investigate the synergistic effect of down-regulation of Livin and Survivin. Methods In the previous experiment ,one pair of the most effective siRNA sequence was chosen from Livin and Survivin siRNA sequences respectively. The two pair of different SiRNA sequences were cloned into one single vector and transformed into DH5α strains. Recombinant expression vector was identified by restriction endonuclease and sequencing analysis. Results It was confirmed by restriction endonuclease and sequencing analysis that multiple siRNA recombinant expression vector targeting Livin and Survivin genes simultaneously was constructed successfully. Conclusion The multiple siRNA recombinant expression vector targeting Livin and Survivin gene simultaneously has been constructed successfully.
