CAJ | 학술논문

[目的]研究试管梨苗嫁接的影响因素及较适合的条件.[方法]以苹果梨、香水梨和山梨种胚培养的组培苗为试材,研究不同的消毒时间、嫁接方法及生长调节剂等因素对其生长的影响.[结果]种子消毒处理及鲜果取种组培苗的成活率均较高,达75.0%以上;以香水梨和苹果梨为接穗,无根山梨为砧木时的嫁接苗成活率有显著差异,其中以香水梨为接穗的成活率较高;以香水梨为接穗,山梨和香水梨为砧木时,带根的材料嫁接时的成活率都显著高于无根嫁接苗的成活率;此外,添加植物生长调节物质可以有效提高嫁接成活率,在培养基MS+BA 1.0 mg/L+IBA 0.3 mg/L中嫁接苗的成活率最高,达86.7%.[结论]该研究可为梨无性系砧木的扩繁推广和作为基砧应用提供了理论依据.
[목적]연구시관리묘가접적영향인소급교괄합적조건.[방법]이평과리、향수리화산리충배배양적조배묘위시재,연구불동적소독시간、가접방법급생장조절제등인소대기생장적영향.[결과]충자소독처리급선과취충조배묘적성활솔균교고,체75.0%이상;이향수리화평과리위접수,무근산리위침목시적가접묘성활솔유현저차이,기중이향수리위접수적성활솔교고;이향수리위접수,산리화향수리위침목시,대근적재료가접시적성활솔도현저고우무근가접묘적성활솔;차외,첨가식물생장조절물질가이유효제고가접성활솔,재배양기MS+BA 1.0 mg/L+IBA 0.3 mg/L중가접묘적성활솔최고,체86.7%.[결론]해연구가위리무성계침목적확번추엄화작위기침응용제공료이론의거.
[Objective]The research aimed to study the influencing factor and adaptive levels of survival rate of grafting and rooting rate of tube pear.[Method]The experiment adopted the test tube plants of Pingguoli,Xiangshuili and Shanli as materials,and the effects of different disinfection time, micro-grafting methods and hormone on its growth were studied. [Result]The results showed that tissue culture seedlings cultured with disinfection seeds and fresh fruit seeds had higher survival rates,which could reached above 75.0%. Taken Pingguoli and Xiangshuili for cions,Shanli without root for rootstock,the survival rate of graft seedlings had obvious difference.And Xiangshuili for cions had higher survival rates.Taken Xiangshuili for cions,Shanli and Xiangshuili for rootstock,the survival rate of graft seedlings with root had higher survival rates than that without root.Added hormone to the media could improve the survival rate of micro-grafting plants, in the medium of MS+BA 1.0 mg/L+IBA 0.3 mg/L,the survival rate reached 86.7%.[Conclusion]This study could provide theoretical basis for the promotion of clonal rootstock of pear and the application as base stock.