CAJ | 학술논문

目的通过检测EMT相关蛋白E-cadherin、Vimentin、a-SMA在前列腺癌组织中的表达及其与病理分级、临床分期的关系,初步判断体内EMT现象与前列腺癌进展是否存在确切的关联.方法采用免疫组织化学方法检测35例正常前列腺组织和43例前列腺癌组织中的上皮标记蛋白E-cadherin与间质标记蛋白Vimentin和a-SMA的表达情况,并与病理分级和临床分期之间的关联作统计学分析.结果 E-cadherin、Vimentin、a-SMA在前列腺癌组织中的表达率分别为48.8%,72.1%,36.5%.这些蛋白的表达与前列腺癌的分化程度,病理分期密切相关(P<0.05),E-cadherin与Vimentin、a-SMA蛋白表达负相关.结论 EMT转化现象是人体内前列腺癌恶性进展的重要原因.这种细胞属性的变化现象与肿瘤的侵袭和预后密切相关.
목적 통과검측EMT상관단백E-cadherin、Vimentin、a-SMA재전렬선암조직중적표체급기여병리분급、림상분기적관계,초보판단체내EMT현상여전렬선암진전시부존재학절적관련.방법 채용면역조직화학방법검측35례정상전렬선조직화43례전렬선암조직중적상피표기단백E-cadherin여간질표기단백Vimentin화a-SMA적표체정황,병여병리분급화림상분기지간적관련작통계학분석.결과 E-cadherin、Vimentin、a-SMA재전렬선암조직중적표체솔분별위48.8%,72.1%,36.5%.저사단백적표체여전렬선암적분화정도,병리분기밀절상관(P<0.05),E-cadherin여Vimentin、a-SMA단백표체부상관.결론 EMT전화현상시인체내전렬선암악성진전적중요원인.저충세포속성적변화현상여종류적침습화예후밀절상관.
Objective To detect the expression of EMT associated proteins including E-cadherin,Vimentin and a-SMA in prostate carcinoma tissues and investigate the relationship between occurrence of EMT and advanced prostate cancer.Methods The expression of E-cadherin,Vimentin and a-SMA in 43 cases of prostate carcinoma was detected by immunohistochemistry,and statistical analysis were used to evaluate the relationship of EMT associated proteins expression and pathological grades or clinical stages.Results The positive expression rates of E-cadherin,Vimentin and a-SMA in prostate carcinoma were 48.8 %,72.1% and 36.5 % respectively.The expression of E-cadherin,Vimentin and a-SMA were positively correlated with differentiation grade,clinical staging and metastasis of prostate carcinoma (P＜0.05).The expression of Vimentin and a-SMA was negatively correlated with that of E-cadherin.Conclusion The expression of EMT associated proteins might be useful markers for prediction of biological behavior and prognosis of prostate carcinoma.