CAJ | 학술논문

目的探讨转录因子FOXO1和FOXO3a在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中的表达水平及其与SLE疾病活动性的关系.方法选择SLE活动性、SLE非活动性患者和正常对照,运用反转录一聚合酶链反应(RT-PCR)和Western blot检测SLE患者和健康对照组PBMC中FOXO1和FOXO3a mRNA及蛋白的表达水平.结果活动性SLE患者PBMC中FOXO1 mRNA和蛋白的表达水平明显低于SLE非活动性患者和正常对照(P<0.05),非活动性SLE患者FOXO1 mRNA和蛋白的表达水平明显低于正常对照(P<0.05).FOX03a mRNA和蛋白的表达水平在3组间差异无统计学意义.FOXO1 mRNA水平与狼疮活动指数(SLEDAI)呈显著负相关(r=-0.651,P<0.05).结论 SLE患者PBMC中FOXO1 mRNA和蛋白的表达水平显著低于健康对照,随着SLE患者病情的好转,FOXO1 mRNA和蛋白的表达水平逐渐增高.FOXO1与SLE疾病的活动性呈负相关.FOXO3a与SLE疾病的活动性无关.
목적 탐토전록인자FOXO1화FOXO3a재계통성홍반랑창(SLE)환자외주혈단개핵세포(PBMC)중적표체수평급기여SLE질병활동성적관계.방법 선택SLE활동성、SLE비활동성환자화정상대조,운용반전록일취합매련반응(RT-PCR)화Western blot검측SLE환자화건강대조조PBMC중FOXO1화FOXO3a mRNA급단백적표체수평.결과 활동성SLE환자PBMC중FOXO1 mRNA화단백적표체수평명현저우SLE비활동성환자화정상대조(P<0.05),비활동성SLE환자FOXO1 mRNA화단백적표체수평명현저우정상대조(P<0.05).FOX03a mRNA화단백적표체수평재3조간차이무통계학의의.FOXO1 mRNA수평여랑창활동지수(SLEDAI)정현저부상관(r=-0.651,P<0.05).결론 SLE환자PBMC중FOXO1 mRNA화단백적표체수평현저저우건강대조,수착SLE환자병정적호전,FOXO1 mRNA화단백적표체수평축점증고.FOXO1여SLE질병적활동성정부상관.FOXO3a여SLE질병적활동성무관.
Objective To study the expression of transcription factor FOXO1 and FOXO3a on peripheral blood mononuclear cells (PBMC) in patients with active or inactive systemic lupus erythematosus (SLE) and investigate the effect of FOXO1 and FOXO3a on the clinical features of SLE. Methods Thirty SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot were applied to analyze the expression of FOXOI and FOXO3a. Results The level of FOXO1 expression was significantly decreased in active SLE patients compared with controls and patients with inactive SLE (P<0.05). The level of FOXO1 expression in inactive SLE patients was lower than that of the controls (P<0.05). The expression of FOXO1 mRNA was negatively correlated to SLEDAI. However, the level of FOXO3a was similar among the three groups. Conclusion The result suggests that FOXO1 may be involved in the pathogenesis of SLE and the expression level of FOXOI may be a good indicator for the disease activity of SLE.