CAJ | 학술논문

目的建立近似人病理生理过程的动物脂肪肝模型,使之适用于影像学定量诊断或药物疗效的验证研究.方法健康新西兰大白兔45只,随机分为四组:正常对照组10只,普通饲料及水喂养9周;实验1、2、3组,各10只,采用高脂高糖饲料+酒精饮料分别喂养3周、6周和12周;补充组5只,给予实验组相同的喂养和处理.采用HE染色和苏丹Ⅳ染色对动物肝脏进行组织学检查.结果实验成模率为87%(39/45),死亡率为13%(6/45),出现正常肝10只,轻度脂肪肝10只,中度脂肪肝9只,重度脂肪肝10只(HE染色),与苏丹Ⅳ染色Vv值分级的诊断符合率约为75%.结论综合运用高脂高糖饲料+酒精饮料可成功建立近似人病理生理过程的动物脂肪肝模型.
목적 건립근사인병리생리과정적동물지방간모형,사지괄용우영상학정량진단혹약물료효적험증연구.방법 건강신서란대백토45지,수궤분위사조:정상대조조10지,보통사료급수위양9주;실험1、2、3조,각10지,채용고지고당사료+주정음료분별위양3주、6주화12주;보충조5지,급여실험조상동적위양화처리.채용HE염색화소단Ⅳ염색대동물간장진행조직학검사.결과 실험성모솔위87%(39/45),사망솔위13%(6/45),출현정상간10지,경도지방간10지,중도지방간9지,중도지방간10지(HE염색),여소단Ⅳ염색Vv치분급적진단부합솔약위75%.결론종합운용고지고당사료+주정음료가성공건립근사인병리생리과정적동물지방간모형.
Objective To establish animal model of fatty liver similar with human pathophysiology used for the study of imaging quantitative diagnosis or verification of medicine curative effect. Methods Forty-five New Zealand white rabbits were used in this study. They were divided randomly into five groups : 10 rabbits in normal control group, 10 rabbits in experiment group 1,10 rabbits in experiment group 2,10 rabbits in experi-ment group 3,and remaining 5 rabbits were in a supplement group. Rabbits in normal control group were given free access to normal food and water for 9 weeks. Rabbits in the experimental groups were fed a high-fat and high-sucrose diet for 3,6,and 12 weeks,respectively. Rabbits in the supplement group were fed the same diet as the experimental groups. Liver specimen stained with HE and Sudan Ⅳ was examined histological. Results The success rate of hepatic steatotie models was 87 % (39 of 45 ) ;mortality was 13 % (6 of 45 ). There were 10 nor-mal livers, 10 mildly fatty livers, 9 moderately fatty livers, and 10 severely fatty livers in results (stained with HE), and the rate of conforming to Sudan Ⅳ was 75%. Conclusion The animal model of fatty liver with differ-ent severity can be established by being fed a high fat, high sucrose and alcohol diet.