CAJ | 학술논문

目的通过观察血吸虫可溶性虫卵抗原(SEA)孵育培养的脑血管内皮细胞缝隙连接(GJ)蛋白的表达和分布，探讨脑动脉GJ在脑血吸虫病病理发生中的作用。方法使用血吸虫SEA孵育培养幼兔脑基底动脉血管内皮细胞，实验分对照组、SEA 1～5组：加入SEA（质量浓度分别为10.0％、5.0％、3.3％、2.5％、2.0％），应用逆转录—聚合酶链式反应(RT-PCR)技术测定兔脑血管内皮细胞GJ蛋白Cx37 mRNA的表达；应用Western 印迹技术测定兔脑血管内皮细胞GJ蛋白Cx37蛋白的表达。结果对照组和SEA 1 ～5组的GJ蛋白Cx37 mRNA水平分别为0.239±0.037、0.260±0.043、0.218±0.310、0.647±0.040、0.419±0.036、0.513±0.038;其中SEA 3～5组的GJ蛋白Cx37 mRNA水平高于对照组（P均＜0.05）。对照组和SEA 1～5组GJ 蛋白Cx37蛋白表达分别为0.401±0.045、0.485±0.048、0.749±0.052、1.119±0.063、1.015±0.057、0.605±0.047,其中SEA 2 ～5组Cx37蛋白表达高于对照组（P均＜0.05）。结论血吸虫SEA孵育培养的兔脑血管内皮细胞GJ蛋白Cx37 mRNA及其蛋白水平高于对照组，提示GJ蛋白在SEA及其分泌物浸润脑动脉沉积于脑组织,从而在诱发脑血吸虫病的病理发生机制中可能起重要的作用。
목적 통과관찰혈흡충가용성충란항원(SEA)부육배양적뇌혈관내피세포봉극련접(GJ)단백적표체화분포，탐토뇌동맥GJ재뇌혈흡충병병리발생중적작용。방법 사용혈흡충SEA부육배양유토뇌기저동맥혈관내피세포，실험분대조조、SEA 1～5조：가입SEA（질량농도분별위10.0％、5.0％、3.3％、2.5％、2.0％），응용역전록—취합매련식반응(RT-PCR)기술측정토뇌혈관내피세포GJ단백Cx37 mRNA적표체；응용Western 인적기술측정토뇌혈관내피세포GJ단백Cx37단백적표체。결과 대조조화SEA 1 ～5조적GJ단백Cx37 mRNA수평분별위0.239±0.037、0.260±0.043、0.218±0.310、0.647±0.040、0.419±0.036、0.513±0.038;기중SEA 3～5조적GJ단백Cx37 mRNA수평고우대조조（P균＜0.05）。대조조화SEA 1～5조GJ 단백Cx37단백표체분별위0.401±0.045、0.485±0.048、0.749±0.052、1.119±0.063、1.015±0.057、0.605±0.047,기중SEA 2 ～5조Cx37단백표체고우대조조（P균＜0.05）。결론 혈흡충SEA부육배양적토뇌혈관내피세포GJ단백Cx37 mRNA급기단백수평고우대조조，제시GJ단백재SEA급기분비물침윤뇌동맥침적우뇌조직,종이재유발뇌혈흡충병적병리발생궤제중가능기중요적작용。
Objective To explore the effects of gap junctional(GJ)proteins in pathogenesis of cerebral schistosomiasis, through observing the expression of gap junctional proteins Cx37 mRNA in cultured cerebral arterial endothelium incubated with soluble eggs antigen(SEA). Methods Cerebral artery endothelial cells of rabbits were incubated with SEA, and the experiments were divided into control group and SEA 1 - 5 groups (SEA concentrations were 10.0％ ,5.0％ ,3.3％ ,2.5％,2.0％, respectively), reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the expression of Cx37 mRNA and protein in cerebral artery endothelial cells of rabbits, respectively. Results Cx37 mRNA levels of control and SEA 1 - 5 groups were 0.239 ± 0.037, 0.260 ± 0.043, 0.218 ± 0.310, 0.647 ± 0.040, 0.419 ± 0.036, and 0.513 ± 0.038, respectively;SEA 3 - 5 groups were higher than control group of mRNA levels(all P＜ 0.05). Cx37 protein levels of control and SEA 1 - 5 group were 0.401 ± 0.045, 0.485 ± 0.048, 0.749 ± 0.052, 1.119 ± 0.063, 1.015 ± 0.057 and 0.605 ±0.047, respectively, of which SEA 2 - 5 groups were higher than control group(all P ＜ 0.05). Conclusions Expression levels of Cx37 mRNA and protein in cultured cerebral artery endothelial cells incubated with SEA are higher than those of control cerebral artery endothelial cells, which suggests that the gap junction proteins may play an important role in pathogenesis of cerebral schistosomiasis through SEA and its secretion in infiltration of brain tissue and deposition in the cerebral arteries.