CAJ | 학술논문

目的观察自体骨髓间充质干细胞移植治疗原发性脑干损伤的近期有效性和安全性.方法 2007年7月至2010年7月我院收治原发性脑干损伤患者54例.移植组30例患者通过蛛网膜下腔注射方式行自体骨髓间充质干细胞移植,选择同时期入院但未行干细胞移植患者24例作为对照组.两组患者移植后1个月进行NIHSS评分,移植后6个月进行疗效比较.同期检测血常规、凝血机制、生化全项、肿瘤标记物.结果移植后1个月,移植组患者NIHSS评分与对照组比较差异有统计学意义[分别为(10.86 ±7.48)、(18.26±8.74)分,t=2.681,P＜0.05]；移植后6个月进行疗效比较差异有统计学意义(Z=2.306,P ＜0.05).随访各项血液检查结果未出现明显异常.结论自体骨髓间充质干细胞移植治疗原发性脑干损伤安全且近期疗效确定,远期疗效尚待进一步观察.
목적 관찰자체골수간충질간세포이식치료원발성뇌간손상적근기유효성화안전성.방법 2007년7월지2010년7월아원수치원발성뇌간손상환자54례.이식조30례환자통과주망막하강주사방식행자체골수간충질간세포이식,선택동시기입원단미행간세포이식환자24례작위대조조.량조환자이식후1개월진행NIHSS평분,이식후6개월진행료효비교.동기검측혈상규、응혈궤제、생화전항、종류표기물.결과 이식후1개월,이식조환자NIHSS평분여대조조비교차이유통계학의의[분별위(10.86 ±7.48)、(18.26±8.74)분,t=2.681,P＜0.05]；이식후6개월진행료효비교차이유통계학의의(Z=2.306,P ＜0.05).수방각항혈액검사결과미출현명현이상.결론 자체골수간충질간세포이식치료원발성뇌간손상안전차근기료효학정,원기료효상대진일보관찰.
Objective To explore the short-term curative effect and safety of autologous bone marrow mesenchymal stem cells transplantation in patients with primary brain stem injury.Methods Fifty-four cases with primary brain stem injury were hospitalized during Jul.2007 to Jul.2010 at Liaocheng Brain Hospital,Shandong Province.All cases were randomized into transplantation group( n =30)or control group( n =24 ).The transplantation group was treated with autologous bone marrow mesenchymal stem cell transplantation by subarachnoid space injection (n =30).The control group were selected from primary brain stem injury patients without stem cell transplantation who were hospitalized at the same period with patients from the transplantation group.Respectively,National Institutes of Health Stroke Scale (NIHSS) score was employed to evaluate the condition of patients in the two groups one month after treatment,and Glasgow Outcome Scale (GOS) score was used to evaluate curative effects of the two groups at sixth months after treatment.Meanwhile,some other parameters were observed,including blood routine,clotting mechanisms,biochemical items and tumor markers.Results There was significant difference between the transplantation group and the control group in N IHSS score at one month after treatment [ ( 10.86 ± 7.48) vs.( 18.26 ± 8.74),t =2.681,P ＜ 0.05 ].GOS score was significantly different( Z =2.306,P ＜ 0.05 ) between the transplantation group and the control group at sixth month after transplantation.There was no significant difference between the two groups in the blood examination results during the followed-up period.Conclusion Autologous bone marrow mesenchymal stem cells transplantation is confirmed to be an effective and safe therapy in patients with primary brain stem injury in the short-term.Further evaluation still needs for its long-term efficacy on primary brain stem injury