CAJ | 학술논문

目的探讨高糖环境下色素上皮衍生因子(PEDF)对大鼠视网膜Müller细胞的影响.方法将体外25 mmoL/L葡萄糖培养的大鼠Müller细胞以100 ng/ml PEDF或10 ng/ml白细胞介素-1β(IL-1β)孵育24 h,采用间接免疫荧光、Western印迹和实时定量PCR检测相关蛋白和mRNA表达,采用MTT检测Müller细胞活性.结果细胞免疫荧光组化、Western印迹和实时定量PCR检测显示在高糖环境下PEDF可以下调Müller细胞IL-1β蛋白和mRNA的表达,同样IL-1β也可以下调Müller细胞PEDF蛋白和mRNA的表达(P＜0.05);PEDF明显提高视网膜Müller细胞活性(0.48±0.09对0.64±0.17,P＜0.05).结论模拟糖尿病状态下,PEDF可以下调Müller细胞中IL-1β的表达,提高Müller细胞活性,可能对糖尿病炎症引起的病理改变起到一定抑制作用.
목적 탐토고당배경하색소상피연생인자(PEDF)대대서시망막Müller세포적영향.방법장체외25 mmoL/L포도당배양적대서Müller세포이100 ng/ml PEDF혹10 ng/ml백세포개소-1β(IL-1β)부육24 h,채용간접면역형광、Western인적화실시정량PCR검측상관단백화mRNA표체,채용MTT검측Müller세포활성.결과세포면역형광조화、Western인적화실시정량PCR검측현시재고당배경하PEDF가이하조Müller세포IL-1β단백화mRNA적표체,동양IL-1β야가이하조Müller세포PEDF단백화mRNA적표체(P＜0.05);PEDF명현제고시망막Müller세포활성(0.48±0.09대0.64±0.17,P＜0.05).결론모의당뇨병상태하,PEDF가이하조Müller세포중IL-1β적표체,제고Müller세포활성,가능대당뇨병염증인기적병리개변기도일정억제작용.
Objective To investigate the effect of pigment epithelium-derived factor (PEDF) on rat retinal Müller cells under high glucose conditions. Methods Müller cells cultured in 25 mmoL/L glncose were incubated with 100 ng/ml PEDF or 10 ng/ml interleukin-1β(IL-1β) for 24 h. The expression of IL-1β or PEDF in Müller cells was measured by indirect immunofluorescence, Western blot or realtime RT-PCR. The survival of Müller cells was detected by MTT assay. Results Under high glucose conditions, expression of IL-1β or PEDF was decreased after treated with 100 ng/ml PEDF or 10 ng/ml IL-1β for 24 hours by the methods of immunocytochemistry, Western blot or realtime PCR (P ＜ 0.05). Activity of Müller cells was increased significantly by PEDF (0.48±0.09 vs 0.64±0.17, P＜0.05). Conclusion In mimic diabetic conditions, PEDF decreases expression of IL-1β in rat retinal Müller cells and enhances the cell activity. To some degree, PEDF may block the process of inflammation in diabetic retinopathy.