CAJ | 학술논문

目的观察心肌非缺血性梗死发生发展的规律.方法中国小型猪12头,体重18～20 kg,实验组结扎LAD上等流量点,对照组解剖相应部位LAD外膜(n=6),术中测定左心室心底和心尖部位血流动力学指标.4周后取出心脏标本,将左心室分成17段,4%多聚甲醛固定1周,苏木素-伊红(HE)、PATH及天狼星红染色进行病理学观察.结果结扎LAD 4周后,左心室17段心肌内均可发现大小不等的梗死灶.左心室腔近心端收缩压收缩压[(264.05±49.34)比(168.35±31.74)mmHg(1 mm Hg=0.133 kPa)]、平均压[(128.65±33.68)比(82.86±14.45)nnnHg、发展压[(255.09±103.18)比(178.70±31.70)nnnHg]与心尖部比较差异有统计学意义(P＜0.05).结论结扎猪左冠状动脉前降支后,左心室非缺血区域可发生局灶性梗死.
목적 관찰심기비결혈성경사발생발전적규률.방법 중국소형저12두,체중18～20 kg,실험조결찰LAD상등류량점,대조조해부상응부위LAD외막(n=6),술중측정좌심실심저화심첨부위혈류동역학지표.4주후취출심장표본,장좌심실분성17단,4%다취갑철고정1주,소목소-이홍(HE)、PATH급천랑성홍염색진행병이학관찰.결과 결찰LAD 4주후,좌심실17단심기내균가발현대소불등적경사조.좌심실강근심단수축압수축압[(264.05±49.34)비(168.35±31.74)mmHg(1 mm Hg=0.133 kPa)]、평균압[(128.65±33.68)비(82.86±14.45)nnnHg、발전압[(255.09±103.18)비(178.70±31.70)nnnHg]여심첨부비교차이유통계학의의(P＜0.05).결론 결찰저좌관상동맥전강지후,좌심실비결혈구역가발생국조성경사.
Objective To observe the development of non-ischemic myocardial infarction.Methods Twelve Chinese mini-pigs,weishing 18-20 kg,were randomly divided into the experimental group and control group(n=6).LAD was ligated at site of(25.06±2.50)ml/min in experimental group and tunica adventitia was dissected free only in control group respectively.All animals underwent serial hemodvnamie studies of apical and bottom of left ventricle during operation.Four weeks after LAD ligation left ventricle myocardium WaS divided into 17 segments.and sections from each myocardium segment were fixed in 5% buffered formalin.paraffin embedded,sectioned at 3 to 4 m,and stained with hematoxylin and eosin.PTAH and Picrosifins Red for pathologic observations.Results In the experimental group,there were significant increases in left ventricle systolic pressure[(264.05±49.34)vs(168.35±31.74)mm Hg(1 mm Hg)=0.133 kPa)],mean.blood pressure[(128.65±33.68)vs(82.86±14.45)mm Hg]and development pressure[(255.09±103.18)vs(178.70±31.70)mm Hg]in the bottom as,conpared with those in the apical of left ventricle 4 weeks after LAD ligation.Analysis of the histologic sections from the 17 segments of LV,including the infarct,border zone and the remote demonstrated fibrosis consistent with healed infarction and invjable tissues occurred in all the 17 segments of myocardium.Conclusion Infracted myocardium extends to involve 17 segments of myocardium of the whole left ventricle as left ventricle remodeling after LAD ligation.