CAJ | 학술논문

目的探讨胎脑组织移植后的存活情况及移植物与受体脑组织之间的神经纤维联系.方法以成年雄性Wistar大鼠为受体,胎龄为15～17天的Wistar胎鼠为供体.将胎鼠的脑皮层组织块移植至受体鼠的损毁脑腔内.术后应用环孢素A预防排斥反应.术后1、3、5个月处死动物,应用免疫组织化学技术(ABC法)进行脑组织切片染色,光镜及电镜下观察.结果同种胎脑皮层组织移植后存活率为30%,5-羟色胺能神经纤维从受体脑组织内长入移植物中,证明二者之间已建立神经纤维联系.结论胎脑移植物与受体脑组织之间可建立神经纤维联系.
목적 탐토태뇌조직이식후적존활정황급이식물여수체뇌조직지간적신경섬유련계.방법 이성년웅성Wistar대서위수체,태령위15～17천적Wistar태서위공체.장태서적뇌피층조직괴이식지수체서적손훼뇌강내.술후응용배포소A예방배척반응.술후1、3、5개월처사동물,응용면역조직화학기술(ABC법)진행뇌조직절편염색,광경급전경하관찰.결과 동충태뇌피층조직이식후존활솔위30%,5-간색알능신경섬유종수체뇌조직내장입이식물중,증명이자지간이건립신경섬유련계.결론 태뇌이식물여수체뇌조직지간가건립신경섬유련계.
Objective To investigate the survival of fetal rat cerebral cortex homografted to adult rat brain and neural connection between recipients brain and grafting tissue.Methods Normal male adult Wistar rats were used as host or recipients,and the donor tissues were obtained from 15 to 17th day fetus of Wistar rats.The donor tissues were taken from the cerebral cortex of fetus and cut into pieces which were transplanted into the brain cavity previously prepared of the recipients.Transplanted rats received daily intraperitoneal iniection of 10 mg/kg cyclosporin A for 2 weeks to prevent immuno-rejection.All the animals were killed at different intervals(1 to 5 months)by perfusing fixative solution transcardially.The sections of rat brain specimens were stained with ABC immunohistochemistry and examined by light and electron microscope.Results Only 30% of the homografts survived and the sereotonergic-like nerve fibers were projected from host brain tissue.Conclusion The grafted tissue of fetal brain cortex might survive and form neural connections with the cerebral cortex of recipients after homograit transplantation.