CAJ | 학술논문

目的观察新型光敏剂M007介导的光动力疗法(PDT)对体外培养的人肝癌SMMC-7721细胞株灭活效果及对残余细胞远期增殖能力的影响.方法以人肝癌SMMC-7721细胞株、光敏剂M007为研究对象,设置不同浓度的剂量组(2、4、16 μmol/L),以630 ～ 660 nm波长的多光源系统光照10 min(光功率密度为0.036 W/cm2、光剂量为2.16 J/cm2)处理1×106人肝癌SMMC-7721细胞,采用流式细胞仪分析细胞死亡率、死亡方式及噻唑蓝(MTT)实验、平板克隆形成实验评估不同组残存细胞的增殖能力.结果 M007浓度为2、4、16 μmol/L时,其介导的光动力反应使人肝癌SMMC-7721细胞死亡率分别达到(68.50±3.33)％、(96.70 ±2.63)％、(99.30 ±0.30)％；2、4和16μmol/L M007-PDT组中的残存细胞生长曲线低平,其吸光度值未随培养时间延长而增加(P＞0.05),4、16 μmol/L未能形成细胞集落,但2μmol/L剂量组可见少量克隆形成.结论 MO07在4、16 μmol/L剂量时,其介导的光动力反应对体外培养的SMMC-7721细胞株有确切的灭活效果,残存细胞无远期增殖能力.
목적 관찰신형광민제M007개도적광동력요법(PDT)대체외배양적인간암SMMC-7721세포주멸활효과급대잔여세포원기증식능력적영향.방법 이인간암SMMC-7721세포주、광민제M007위연구대상,설치불동농도적제량조(2、4、16 μmol/L),이630 ～ 660 nm파장적다광원계통광조10 min(광공솔밀도위0.036 W/cm2、광제량위2.16 J/cm2)처리1×106인간암SMMC-7721세포,채용류식세포의분석세포사망솔、사망방식급새서람(MTT)실험、평판극륭형성실험평고불동조잔존세포적증식능력.결과 M007농도위2、4、16 μmol/L시,기개도적광동력반응사인간암SMMC-7721세포사망솔분별체도(68.50±3.33)％、(96.70 ±2.63)％、(99.30 ±0.30)％；2、4화16μmol/L M007-PDT조중적잔존세포생장곡선저평,기흡광도치미수배양시간연장이증가(P＞0.05),4、16 μmol/L미능형성세포집락,단2μmol/L제량조가견소량극륭형성.결론 MO07재4、16 μmol/L제량시,기개도적광동력반응대체외배양적SMMC-7721세포주유학절적멸활효과,잔존세포무원기증식능력.
Objective To investigate the effect of new photosensitizer M007-mediated photodynamic therapy on human hepatoma SMMC-7721 cells in vitro and proliferation capacity of the residuai cells.Methods Subcultured SMMC-7721 human hepatocellular carcinoma cells lines,methylene blue derivatives M007 as the photosensitizer,setting different concentrations of M007-PDT groups (2,4,16 μmol/L),multiphoto source system illuminates 1 × 106 cells in 630-660 nm wave length for 10 min (luminous power density is 0.036 W/cm2,the light dosage is 2.16J/cm2 ),analyzing cell mortality and manner of death by flow cytometry,Methyl thiazol tetrazolium (MTT) test and colony formation assay to assess residual cell proliferation.Results When the M007 concentration in 2,4,16 μmol/L,the cell mortality in M007-PDT groups were (68.50 ± 3.33 ) ％,(96.70 ± 2.63 ) ％,(99.30 ± 0.30 ) ％ respectively ; in 2,4 and 6 μmol/L M007-PDTgroups,residual cell growth curve is low and flat,and the optical density value is not increased with the incubation time(P ＞ 0.05),4,16 μmoI/L M007-PDT groups failed to form a colony,but 2 μmol/L M007-PDT group had a few colony formation occasionally.Conclusion M007 doses in 4,16 μmol/L,M007 mediated photodynamic therapy on SMMC-7721 cell line has the exactly inactivated effect,and the residual cells lost long-term proliferative capacity in vitro.